Abstract:

Tobacco （ Nicotiana tabacum L.） pedicel in the blooming period was used to study the distribution of auxin binding protein 1 (ABP1) in the tobacco tissue and cell at different time in the culture of thin cell layers (TCL). Using the immunofluorescence marker it was indicated that the main ABP1 was distributed in the epidermis and the 1st and 2nd layers of the subepidermis cells. A little ABP1 was distributed in the cortex. Tobacco ABP1 was induced to express in the protoplast of tobacco pedicel TCL cultured in MS culture medium containing IAA and BA. Expression of ABP1 in the protoplast was stronger in the active period of vegetative bud differentiation. ABP1 expression became weaker in later period of differentiation. The result of SDS-PAGE and Western blotting showed the molecular weight of tobacco ABP1 in TCL was 26 kD.

烟草薄层培养生长素结合蛋白ABP1 的定位和ABP1在细胞分化中的变化
高启祥 李颖章*  刘淑兰

（中国农业大学生物学院，农业部植物生理生化重点开放实验室，北京100094）

摘要：以烟草(Nicotiana tabacum L.)盛花期花梗薄层为材料, 研究营养芽分化的不同时期生长素结合蛋白(ABP1)在组织与细胞中的分布变化.免疫荧光标记结果表明,烟草花梗中ABP1主要分布于表皮及亚表皮1～2层细胞内.不同分化期ABP1在烟草花梗薄层原生质体中的表达不同,细胞分化旺盛期ABP1的表达最强,分化后期ABP1的表达有所减弱; Western blotting结果表明,ABP1多克隆抗血清与烟草花梗薄层细胞及分化过程中26 kD蛋白有免疫交叉反应.

关键词： 烟草；原生质体；生长素结合蛋白（ABP1）