Abstract:

The relationship between hydrogen peroxide (H2O2) and endopeptidase（EP） in wheat (Triticum aestivum L. cv. Yanmai 158) leaves was studied during natural and artificial aging. Rapid accumulation of endogenous H2O2 and marked increase of EP activity were observed during the later phase of aging. A new EP isozyme with higher activity was detected by electrophoresis on polyacrylamide gels containing denatured heamoglobin. With the increase of exogenous H2O2, the activity of EP increased at first and then decreased.

小麦叶片老化期间的内肽酶与H₂0₂的关系
张志刚 芮琪 徐朗莱
（南京农业大学理学院，南京210095）

摘要： 研究了H₂0₂和蛋白水解酶在小麦 (Triticumaestivum L .cv.Yanmai15 8)叶片老化过程中的关系。小麦叶片老化期间 ,H₂0₂含量高的叶片中内肽酶活力也高。老化后期 ,内源H₂0₂迅速累积 ,内肽酶活力迅速上升 ;通过内肽酶同工酶电泳可检测到新增一种活力较强的内肽酶。用外源H₂0₂处理全展旗叶的内肽酶粗提液 ,随着H₂0₂浓度的升高 ,内肽酶活力先上升后下降。

关键词： 内肽酶；H₂0₂；小麦叶片；老化

Key words: endopeptidase, H2O2, wheat leaf, aging