Abstract:

The relationship between superoxide and singlet oxygen in PSⅡ particle of spinach under strong illumination was studied with spin-trap ESR technique. The generation of superoxide was increased under D2 environment which prolonged the half life of singlet oxygen. The generation of superoxide was decreased when histidine existed as a scavenger of singlet oxygen. It is highly possible that the superoxide generated in PSⅡ particle originates from singlet oxygen.

光系统II颗粒内单线态氧诱导产生超氧阴离子自由基
刘科1孙健1,2徐英凯1  陈中伟1 张启元1 张兴康1 匡廷云2  刘扬1*

（1. 中国科学院化学研究所，北京100080；2.  中国科学院植物研究所，北京100093）
关键词： 超氧阴离子自由基；单线态氧；PSII；活性氧；自旋捕捉-电子自旋共振技术

Key words: superoxide, singlet oxygen, PSⅡ, reactive oxygen species, spin trapping-ESR