Abstract:

In order to investigate the morphological response of freshwater green algae toelevated CO2 concentration, Chlamydomonas reinhardtii Dang and Scenedesmus obliquus Kütz were cultured with enriched CO2, and their microstructure and ultrastructure were examined by microscopy and electron microscopy. The effect of CO2 enrichment to 186 μmol/L was insignificant on the shape and size of C. reinhardtii, but significant in reducing the volume of S. obliquus. High CO2 increased the amount of chloroplast. The pyrenoids occurred in low CO2 grown cells but not in high CO2 grown ones and more starch granules were observed in the former.

CO2 浓度变化对两种淡水绿藻的显微结构和超微结构的影响

夏建荣¹  高坤山^1,2

(1.汕头大学海洋生物研究所,汕头,515063；2.中国科学院水生生物研究所，武汉, 430072)

为了探讨淡水绿藻在适应CO2浓度变化过程中细胞形态和结构的变化,通过普通显微镜和电子显微镜观察了在不同CO2浓度培养下的莱因衣藻(Chlamydomonas reinhardtii Dang)和斜生栅藻(Scenedesmus obliquus Kütz)细胞.结果表明,CO2浓度变化对莱因衣藻细胞体积没有明显的影响,但斜生栅藻在低浓度CO2培养下细胞体积明显增大,并可见细胞内含有大量颗粒.两种绿藻细胞的超微结构显示,在低浓度CO2培养下,细胞内叶绿体数目明显减少,并可见明显的淀粉盘包围的蛋白核;细胞内还可见大量的淀粉粒.而在高浓度CO2培养下,这两种绿藻细胞内均未见明显的蛋白核和大量淀粉粒出现.

关键词 莱因衣藻 CO2 显微结构 蛋白核 斜生栅藻 淀粉粒 超微结构

Key words: Chlamydomonas reinhardtii, CO2, microstructure, pyrenoid, Scenedesmus obliquus, starch granules, ultrastructure