Abstract:

A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymusracemosus Lam.,Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C.Koch respectively was made. In the course of germplasm development, genome analysis by means of chromosome banding, genomic in situ hybridization (GISH) or fluorescence in situ hybridization (FISH), molecular markers, particularly restriction fragment length polymorphism （RFLP） coupled with aneuploid analysis was employed for the purpose of improving breeding efficiency. Potential use of such germplasm in wheat breeding practice, basic studies and some related problems were also discussed.

基因组分析与小麦抗病育种
刘大钧
（南京农业大学细胞遗传研究所，南京210095）

摘要： 系统总结了南京农业大学细胞遗传研究所近20多年来利用基因组分析方法培育从簇毛麦(Haynaldia villosa Sch.)、大赖草(Leymus racemosus Lam.)、鹅观草(Roegneria kamoji C. Koch)和纤毛鹅观草(R. ciliaris (Trin.) Nevski)导入白粉病和赤霉病抗性的小麦种质的研究进展.利用染色体C-分带、基因组原位杂交、分子标记(特别是RFLP)等技术与非整倍体分析相结合对所创制的种质进行了系统分析与鉴定.还对所培育的小麦种质在育种实践和理论研究中的潜在价值及相关问题进行了讨论.

关键词： 小麦；小麦近缘种；白粉病抗性；赤霉病抗性；染色体分带；原位杂交；分子标记；基因组分析

Key words: Triticum aestivum, wheat relatives, powdery mildew resistance, scab resistance, chromosome banding, in situ hybridization, molecular marker, genome analysis