Abstract:

It was substantiated by morphological observation, chromosome and DNA identification that the bargene, conferring the resistance to herbicide basta in transgenic Chinese cabbage (Brassica campestris ssp. chinensis var. utilisTsen et Lee), could intrude into the genomes of turnip (B. campestris ssp.rapifera), heading Chinese cabbage (B. campestris ssp. pekinensis) and other non_heading Chinese cabbage (B. campestris ssp. chinensis) in a relatively high frequency while into genome of B. napusat a low frequency via natural cross_pollination in open field. Herbicide resistant hybrids of B. nigra, B. carinata, andB. juncea could be obtained at certain frequency in the greenhouse by hand pollination. At no time did the resistant hybrid plants of transgenic Chinese cabbage with B. oleracea, R. sativus and seven wild weeds, widespread in China, gained in our experiments.

传粉导致的转基因白菜与其近缘种属材料间的基因流动
刘凡  赵泓  姚磊  张军杰  曹鸣庆
（1. 北京蔬菜研究中心，北京100089; 北京农业生物技术中心，北京100089）

摘要： 从形态、染色体及分子水平上证实，转基因不结球白菜(Brassica campestris ssp．chinensis var．utilis Tsen et  Lee)中编码除草剂Basta抗性的bar基因能在田间条件下，经自然传粉，以较高频率侵入芜菁(B．campestris ssp．rapifera)、结球白菜(B．campestris ssp．peknensis)和不结球白菜(B．campestrs ssp．chnensis)的基因组中，也能少量侵入同属异种的甘蓝型油菜(B.napus)基因组中；在温室人工辅助授粉条件下，除在上述种中的基因漂移率提高外，bar基因尚能以一定频率侵入同属的黑芥(B．nigra)、埃塞俄比亚芥(B．carnata)、芥菜(B．juncea)基因组中，但始终未能得到转基因白菜与结球甘蓝(B．oleracea)、萝卜(R．sativus)的杂种。转基因白菜与十字花科的7种常见杂草经温室人工辅助授粉，也均未得到抗性杂种。