Abstract:

Phosphorus is one of the three essential macroelements for plant growth. Plants respond to phosphorus starvation through adaptive mechanisms in volved in morphological, biochemical and molecular changes. To investigate the molecular background of the adaptive mechanisms, the suppression subtrac tive hybridization (SSH) method was used to construct a rice phosphorus-starvati on (Pi-starvation) induced cDNA library. Through screening of the cDNA library and sequencing of the enriched cDNAs, 18 known genes and 47 novel genes were identified. The known genes are involved in different metabolic processes, including phosphate uptake and transport, signal transduction, protein synthesis and de gradation, carbon metabolism and stress response. Northern analysis was perform ed to detect the expression patterns of some known genes and novel genes under different phosphorus levels. Different expression patterns of the selected genes were identified, which suggests that genes involved in different pathways may have different responses to Pi-starvation.

利用抑制性扣除杂交技术克隆水稻磷饥饿诱导基因
夏 铭 王首锋 王小兵 李海波 吴平
（浙江大学生命科学学院，杭州310029）

摘要： 磷素是植物生长所必需的重要元素.在缺磷环境中,植物能够调节自身的形态、生理生化和基因表达水平来适应环境的变化.为研究水稻(Oryza sativa L.)耐低磷胁迫的分子机理,采用抑制性扣除杂交技术(SSH)构建磷饥饿诱导的水稻根系扣除cDNA文库.通过文库筛选和测序获得18个已知基因和47个功能未知基因.这些基因参与了不同的代谢过程,包括磷吸收和转运、信号传导、蛋白质合成和降解、碳水化合物代谢和胁迫反应.Northern杂交结果表明,在磷饥饿胁迫下这些基因呈现不同的表达模式,并且不同代谢过程中的基因对磷饥饿有着不同的反应.

关键词： 磷饥饿；扣除杂交（SSH）；基因表达；水稻

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