Abstract:

hloroplast microsatellite primers of wheat (Triticum aestivum L.) and random primers were employed to identify the chloroplast and nuclear genomes of three selfed linesⅡ-2, Ⅱ-Ⅰ-8 (F₂-F₆) and 8-1 (F₃-F₆) ( segregated from Ⅱ-Ⅰ-8 of F₂), which were derived from the same asymmetric somatic hybrid clone between Triticum aestivum L. cv. Jinan 177 and Agropyron elongatum (Host) Nevski. The results showed that the chloroplast genomic components of the three lines were consistent and dominated by that of wheat. Bands characteristic to both parents were only detected in the sequence of the intergenic region between rpl14 and rpl16 of the chloroplast genome, suggesting the existence of chloroplast DNA of A. elongatum in those hybrids. Furthermore, this exogenous integration of chloroplast DNA was passed to F₆ stably. RAPD analysis showed that there were different DNA fragments of A. elongatum in different lines. However, the nuclear genome basically remained stable during passage.

Key words: somatic hybrid lines between Triticum aestivum and Agropyron elongatum , nuclear genome, chloroplast genome , chloroplast microsatellite, RAPD