J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (12): 2396-2410.DOI: 10.1111/jipb.13378

• • 上一篇    下一篇

  

  • 收稿日期:2022-07-04 接受日期:2022-10-02 出版日期:2022-12-01 发布日期:2022-12-30

Chromatin architectural alterations due to null mutation of a major CG methylase in rice

Jinbin Wang1†, Xiaochong Li1†, Qianli Dong1†, Changping Li1, Juzuo Li1, Ning Li1, Baoxu Ding1, Xiaofei Wang1,2,3, Yanan Yu1, Tianya Wang1, Zhibin Zhang1, Yiyang Yu1, Man Lang1, Zixian Zeng4*, Bao Liu1* and Lei Gong1*   

  1. 1 Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun 130024, China
    2 State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    3 Hainan Yazhou Bay Seed Lab, Sanya 572025, China
    4 Department of Biological Science, College of Life Science, Sichuan Normal University, Chengdu 610101, China

    †These authors contributed equally to this work.
    *Correspondences: Zixian Zeng (zengzixian@sicnu.edu.cn); Bao Liu (baoliu@nenu.edu.cn); Lei Gong (gongl100@nenu.edu.cn, Dr Gong is fully responsible for the distribution of the materials associated with this article)
  • Received:2022-07-04 Accepted:2022-10-02 Online:2022-12-01 Published:2022-12-30

Abstract:

Associations between 3D chromatin architectures and epigenetic modifications have been characterized in animals. However, any impact of DNA methylation on chromatin architecture in plants is understudied, which is confined to Arabidopsis thaliana. Because plant species differ in genome size, composition, and overall chromatin packing, it is unclear to what extent findings from A. thaliana hold in other species. Moreover, the incomplete chromatin architectural profiles and the low-resolution high-throughput chromosome conformation capture (Hi-C) data from A. thaliana have hampered characterizing its subtle chromatin structures and their associations with DNA methylation. We constructed a high-resolution Hi-C interaction map for the null OsMET1-2 (the major CG methyltransferase in rice) mutant (osmet1-2) and isogenic wild-type rice (WT). Chromatin structural changes occurred in osmet1-2, including intra-/inter-chromosomal interactions, compartment transition, and topologically associated domains (TAD) variations. Our findings provide novel insights into the potential function of DNA methylation in TAD formation in rice and confirmed DNA methylation plays similar essential roles in chromatin packing in A. thaliana and rice.

[an error occurred while processing this directive]