J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (12): 2425-2437.DOI: 10.1111/jipb.13387

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  • 收稿日期:2022-06-25 接受日期:2022-10-10 出版日期:2022-12-01 发布日期:2022-12-30

High auxin stimulates callus through SDG8-mediated histone H3K36 methylation in Arabidopsis

Jun Ma1,2†, Qiang Li2,3†, Lei Zhang1,2, Sen Cai4, Yuanyuan Liu4, Juncheng Lin2, Rongfeng Huang1,2, Yongqiang Yu1,2, Mingzhang Wen2,5* and Tongda Xu1,2*   

  1. 1 College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    2 Plant Synthetic Biology Center, FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    3 Shanghai Center for Plant Stress Biology, Centre for Excellence in Molecular Plant Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China
    4 Basic Forestry and Proteomics Research Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    5 Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300072, China

    † These authors contributed equally to this work.
    * Correspondences: Mingzhang Wen (wenmz@tju.edu.cn); Tongda Xu (tdxu@sibs.ac.cn, Dr. Xu is fully responsible for the distribution of all materials associated with this article)
  • Received:2022-06-25 Accepted:2022-10-10 Online:2022-12-01 Published:2022-12-30

Abstract:

Callus induction, which results in fate transition in plant cells, is considered as the first and key step for plant regeneration. This process can be stimulated in different tissues by a callus-inducing medium (CIM), which contains a high concentration of phytohormone auxin. Although a few key regulators for callus induction have been identified, the multiple aspects of the regulatory mechanism driven by high levels of auxin still need further investigation. Here, we find that high auxin induces callus through a H3K36 histone methylation-dependent mechanism, which requires the methyltransferase SET DOMAIN GROUP 8 (SDG8). During callus induction, the increased auxin accumulates SDG8 expression through a TIR1/AFBs-based transcriptional regulation. SDG8 then deposits H3K36me3 modifications on the loci of callus-related genes, including a master regulator WOX5 and the cell proliferation-related genes, such as CYCB1.1. This epigenetic regulation in turn is required for the transcriptional activation of these genes during callus formation. These findings suggest that the massive transcriptional reprogramming for cell fate transition by auxin during callus formation requires epigenetic modifications including SDG8-mediated histone H3K36 methylation. Our results provide insight into the coordination between auxin signaling and epigenetic regulation during fundamental processes in plant development.

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