J Integr Plant Biol ›› 2024, Vol. 66 ›› Issue (5): 897-908.DOI: 10.1111/jipb.13648

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  • 收稿日期:2023-11-16 接受日期:2024-03-01 出版日期:2024-05-01 发布日期:2024-05-31

UVR8-TCP4-LOX2 module regulates UV-B tolerance in Arabidopsis

Cheng Li1, 2†, Jiancan Du1, 3†, Huini Xu4, Zhenhua Feng1, Caspar C. C. Chater5, Yuanwen Duan1*, Yongping Yang1* and Xudong Sun1*   

  1. 1. Yunnan Key Laboratory of Crop Wild Relatives Omics, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China
    4. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650031, China
    5. Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AE, UK
    †These authors contributed equally to this work.
    *Correspondences:Yuanwen Duan (duanyw@mail.kib.ac.cn); Yongping Yang (yangyp@mail.kib.ac.cn); Xudong Sun (sunxudong@mail. kib.ac.cn, Dr. Sun is fully responsible for the distributions of all materials associated with this article)
  • Received:2023-11-16 Accepted:2024-03-01 Online:2024-05-01 Published:2024-05-31

Abstract: The phytohormone jasmonate (JA) coordinates stress and growth responses to increase plant survival in unfavorable environments. Although JA can enhance plant UV-B stress tolerance, the mechanisms underlying the interaction of UV-B and JA in this response remain unknown. In this study, we demonstrate that the UV RESISTANCE LOCUS 8 - TEOSINTE BRANCHED1, Cycloidea and PCF 4 - LIPOXYGENASE2 (UVR8-TCP4-LOX2) module regulates UV-B tolerance dependent on JA signaling pathway in Arabidopsis thaliana. We show that the nucleus-localized UVR8 physically interacts with TCP4 to increase the DNA-binding activity of TCP4 and upregulate the JA biosynthesis gene LOX2. Furthermore, UVR8 activates the expression of LOX2 in a TCP4-dependent manner. Our genetic analysis also provides evidence that TCP4 acts downstream of UVR8 and upstream of LOX2 to mediate plant responses to UV-B stress. Our results illustrate that the UV-B-dependent interaction of UVR8 and TCP4 serves as an important UVR8-TCP4-LOX2 module, which integrates UV-B radiation and JA signaling and represents a new UVR8 signaling mechanism in plants.

Key words: anthocyanin accumulation, jasmonic acid, TCP4, UV-B, UVR8

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