J Integr Plant Biol ›› 2024, Vol. 66 ›› Issue (6): 1106-1125.DOI: 10.1111/jipb.13654

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  • 收稿日期:2023-12-12 接受日期:2024-03-13 出版日期:2024-06-01 发布日期:2024-06-25

A signaling cascade mediating fruit trait development via phosphorylation-modulated nuclear accumulation of JAZ repressor

Wei Wang1, Jinyao Ouyang1, Yating Li1, Changsheng Zhai1, Bing He1, Huahan Si1, Kunsong Chen2, Jocelyn K.C. Rose3 and Wensuo Jia1,4*   

  1. 1. College of Horticulture, China Agricultural University, Beijing 100193, China;
    2. College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China;
    3. Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca14853, NY, USA;
    4. Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, China
    *Correspondence: Wensuo Jia (jiaws@cau.edu.cn)
  • Received:2023-12-12 Accepted:2024-03-13 Online:2024-06-01 Published:2024-06-25

Abstract: It is generally accepted that jasmonate-ZIM domain (JAZ) repressors act to mediate jasmonate (JA) signaling via CORONATINE-INSENSITIVE1 (COI1)-mediated degradation. Here, we report a cryptic signaling cascade where a JAZ repressor, FvJAZ12, mediates multiple signaling inputs via phosphorylation-modulated subcellular translocation rather than the COI1-mediated degradation mechanism in strawberry (Fragaria vesca). FvJAZ12 acts to regulate flavor metabolism and defense response, and was found to be the target of FvMPK6, a mitogen-activated protein kinase that is capable of responding to multiple signal stimuli. FvMPK6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues, thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2, which acts to control the expression of lipoxygenase 3 (FvLOX3), an important gene involved in JA biosynthesis and a diverse array of cellular metabolisms. Our data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.

Key words: defense response, JAZ repressor, phosphorylation, strawberry, subcellular translocation

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