J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (2): 375-390.DOI: 10.1111/jipb.13779  cstr: 32098.14.jipb.13779

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  • 收稿日期:2023-10-30 接受日期:2024-09-02 出版日期:2025-02-01 发布日期:2025-02-13

Light signaling-dependent regulation of plastid RNA processing in Arabidopsis

Lili Hu1,2†, Qian Wu1†, Chunyu Wu1, Chunmei Zhang2, Ziying Wu1, Meihui Shi1, Man Zhang1, Sujuan Duan1, Hong‐Bin Wang1,3* and Hong‐Lei Jin1,4,5*   

  1. 1. Institute of Medical Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
    2. School of Life Sciences, Sun Yat‐sen University, Guangzhou 510275, China
    3. Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education, Guangzhou 510006, China
    4. Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510375, China
    5. State Key Laboratory of Traditional Chinese Medicine Syndrome, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

    †These authors contributed equally to this work.
    *Correspondences: Hong‐Bin Wang (wanghongbin@gzucm.edu.cn); Hong‐Lei Jin (jinhl@gzucm.edu.cn, Dr. Jin is fully responsible for the distributions of all materials associated with this article)
  • Received:2023-10-30 Accepted:2024-09-02 Online:2025-02-01 Published:2025-02-13
  • Supported by:
    This work was supported by grants from the National Natural Science Foundation of China (32322007, U22A20446, 32100192) and from the Talent Support Project of Guangdong (2019TQ05N182).

Abstract: Light is a vital environmental signal that regulates the expression of plastid genes. Plastids are crucial organelles that respond to light, but the effects of light on plastid RNA processing following transcription remain unclear. In this study, we systematically examined the influence of light exposure on plastid RNA processing, focusing on RNA splicing and RNA editing. We demonstrated that light promotes the splicing of transcripts from the plastid genes rps12, ndhA, atpF, petB, and rpl2. Additionally, light increased the editing rate of the accD transcript at nucleotide 794 (accD-794) and the ndhF transcript at nucleotide 290 (ndhF-290), while decreasing the editing rate of the clpP transcript at nucleotide 559 (clpP-559). We have identified key regulators of signaling pathways, such as CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), ELONGATED HYPOCOTYL 5 (HY5), and PHYTOCHROME-INTERACTING FACTORs (PIFs), as important players in the regulation of plastid RNA splicing and editing. Notably, COP1 was required for GENOMES UNCOUPLED1 (GUN1)-dependent repression of clpP-559 editing in the light. We showed that HY5 and PIF1 bind to the promoters of nuclear genes encoding plastid-localized RNA processing factors in a light-dependent manner. This study provides insight into the mechanisms underlying light-mediated post-transcriptional regulation of plastid gene expression.

Key words: Light, plastid, post‐transcriptional regulation, RNA editing, RNA splicing

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