J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (2): 226-242.DOI: 10.1111/jipb.13829

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  • 收稿日期:2024-05-30 接受日期:2024-12-09 出版日期:2025-02-01 发布日期:2025-02-13

Tolerance to multiple abiotic stresses is mediated by interacting CNGC proteins that regulate Ca2+ influx and stomatal movement in rice

Lilin Luo1, Yongmei Cui1,2, Nana Ouyang1, Shuying Huang1, Xiaoli Gong1, Lihui Wei3, Baohong Zou1, Jian Hua4* and Shan Lu1*   

  1. 1. State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
    2. Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China
    3. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
    4. Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853, USA

    *Correspondences: Jian Hua (jh299@cornell.edu); Shan Lu (lushan@njau.edu.cn, Dr. Lu is fully responsible for the distributions of all materials associated with this article)
  • Received:2024-05-30 Accepted:2024-12-09 Online:2025-02-01 Published:2025-02-13
  • Supported by:
    This work was supported by grants from the National Natural Science Foundation of China (32370309), the STI 2030‐Major Projects (2023ZD040710X), the National Natural Science Foundation of China (31700223), Jiangsu Agricultural Science and Technology Innovation Fund (CX (23)1033), Jiangsu Collaborative Innovation Center for Modern Crop Production and Cyrus Tang Innovation Center for Crop Seed Industry.

Abstract: Members of the cyclic nucleotide-gated channel (CNGC) proteins are reportedly involved in a variety of biotic and abiotic responses and stomatal movement. However, it is unknown if and how a single member could regulate multiple responses. Here we characterized three closely related CNGC genes in rice, OsCNGC14, OsCNGC15 and OsCNGC16, to determine whether they function in multiple abiotic stresses. The loss-of-function mutants of each of these three genes had reduced calcium ion (Ca2+) influx and slower stomatal closure in response to heat, chilling, drought and the stress hormone abscisic acid (ABA). These mutants also had reduced tolerance to heat, chilling and drought compared with the wild-type. Conversely, overexpression of OsCNGC16 led to a more rapid stomatal closure response to stresses and enhanced tolerance to heat, chilling and drought. The tight association of stomatal closure and stress tolerance strongly suggests that tolerance to multiple abiotic stresses conferred by these OsCNGC genes results at least partially from their regulation of stomatal movement. In addition, physical interactions were observed among the three OsCNGC proteins but not with a distantly related CNGC, suggesting the formation of hetero-oligomers among themselves. This study unveils the crucial role of OsCNGC14, 15 and 16 proteins in stomatal response and tolerance to multiple stresses, suggesting a mechanism of tolerance to multiple stresses that involves calcium influx and stomatal movement regulation.

Key words: ABA, abiotic stress, Ca2+, CNGC, rice, stomata

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