J Integr Plant Biol ›› 2025, Vol. 67 ›› Issue (7): 1805-1822.DOI: 10.1111/jipb.13893

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  • 收稿日期:2024-02-05 接受日期:2025-02-25 出版日期:2025-07-01 发布日期:2025-07-07

Orchestration of leaf curvature by the SBP transcription factor SPL10–REVOLUTA module in Arabidopsis

Pengfei Xu1,2†, Qihui Wan2,3†, Wenna Shao1†, You Wu1†, Feijie Wu2, Xiaorong Li2, Wenqing Ren4, Yuke He2,3*, Shuxia Li5* and Xiang Yu1*   

  1. 1. Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
    2. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, the Chinese Academy of Sciences, Shanghai 200032, China
    3. University of the Chinese Academy of Sciences, Beijing 100040, China
    4. School of Medicine, Tongji University Cancer Center, Shanghai 200092, China
    5. National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, China

    †These authors contributed equally to this work.
    *Correspondences: Shuxia Li (lishuxia@itbb.org.cn); Yuke He (heyk@sippe.ac.cn); Xiang Yu (yuxiang2021@sjtu.edu.cn, Dr. Xiang Yu is fully responsible for the distribution of all materials associated with this article)
  • Received:2024-02-05 Accepted:2025-02-25 Online:2025-07-01 Published:2025-07-07
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (Grant Nos. 32170581 and 32370587 to X.Y., No. 32272043 and No. 32472190 to S.L.), China Postdoctoral Science Foundation (2022M72211 to P.X.) and this research is funded by the Hong Kong Scholars Program.

Abstract: Leaf curvature significantly contributes to important economic traits in vegetable crops. The upward-curling leaf phenotype has been consistently observed upon overexpression of a miR156/157-resistant version of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 10 (SPL10) transcription factor (rSPL10). However, the role of SPL10 in regulating leaf curvature has not been well characterized. In this study, using DNA affinity purification sequencing followed by transient transactivation assays, we found that SPL10 can bind to the promoter and gene body of REVOLUTA (REV), augmenting its expression. The rSPL10 rev-6 double mutant plant displayed a downward-curling leaf phenotype similar to the rev-6 plant, supporting the notion that REV functions downstream of SPL10. Importantly, the SPL10 protein physically interacts with the REV protein, which attenuates the expression of REV promoted by SPL10, leading to the downregulation of REV-regulated genes involved in leaf curvature, such as HB2 and HB4. These findings suggest that the SPL10–REV module acts as a molecular rheostat to prevent excessive amplification of REV transcripts in Arabidopsis. Furthermore, overexpression of the BrpREV1 gene in Chinese cabbage caused the transformation of rosette leaves from flat to upward-curving and accelerated heading. Taken together, our findings reveal the role of SPL10–REV module in orchestrating leaf curvature, which could potentially be utilized for molecular breeding of economical traits in vegetable crops.

Key words: Arabidopsis, Chinese cabbage, leaf curvature, REV, SPL10

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