J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (1): 56-72.DOI: 10.1111/jipb.13193

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  • 收稿日期:2021-08-21 接受日期:2021-11-21 出版日期:2022-01-01 发布日期:2022-01-25

Arabidopsis F-BOX STRESS INDUCED 4 is required to repress excessive divisions in stomatal development

Yi Li1,2, Shan Xue1,3, Qixiumei He1,2, Junxue Wang1,4, Lingling Zhu1,2, Junjie Zou1,5, Jie Zhang1,2, Chaoran Zuo1,2, Zhibin Fan1,2, Junling Yue1,2, Chunxia Zhang1, Kezhen Yang1* and Jie Le1,2*   

  1. 1 Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
    3 The Institute of Scientific and Technical Information of China, Beijing 100038, China
    4 Wenbo School, Jinan 250100, China
    5 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    *Correspondences: Kezhen Yang (ykzdsp@163.com); Jie Le (lejie@ibcas.ac.cn, Dr. Le is fully responsible for the distributions of the materials associated with this article)
  • Received:2021-08-21 Accepted:2021-11-21 Online:2022-01-01 Published:2022-01-25

Abstract: During the terminal stage of stomatal development, the R2R3-MYB transcription factors FOUR LIPS (FLP/MYB124) and MYB88 limit guard mother cell division by repressing the transcript levels of multiple cell-cycle genes. In Arabidopsis thaliana possessing the weak allele flp-1, an extra guard mother cell division results in two stomata having direct contact. Here, we identified an ethylmethane sulfonate-mutagenized mutant, flp-1 xs01c, which exhibited more severe defects than flp-1 alone, producing giant tumor-like cell clusters. XS01C, encoding F-BOX STRESS-INDUCED 4 (FBS4), is preferentially expressed in epidermal stomatal precursor cells. Overexpressing FBS4 rescued the defective stomatal phenotypes of flp-1 xs01c and flp-1 mutants. The deletion or substitution of a conserved residue (Proline166) within the F-box domain of FBS4 abolished or reduced, respectively, its interaction with Arabidopsis Skp1-Like1 (ASK1), the core subunit of the Skp1/Cullin/F-box E3 ubiquitin ligase complex. Furthermore, the FBS4 protein physically interacted with CYCA2;3 and induced its degradation through the ubiquitin-26S proteasome pathway. Thus, in addition to the known transcriptional pathway, the terminal symmetric division in stomatal development is ensured at the post-translational level, such as through the ubiquitination of target proteins recognized by the stomatal lineage F-box protein FBS4.

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