J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (1): 73-86.DOI: 10.1111/jipb.13195

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  • 收稿日期:2021-07-07 接受日期:2021-11-22 出版日期:2022-01-01 发布日期:2022-01-25

The role of senescence-associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar

Hui He1 , Xue‐Qin Song1 , Cheng Jiang2 , Ying‐Li Liu1 , Dian Wang3 , Shuang‐Shuang Wen1 , Guo‐Hua Chai4 , Shu‐Tang Zhao1,5* and Meng‐Zhu Lu1,2,5*   

  1. 1 State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
    2 State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China
    3 College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China
    4 College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, China
    5 Co‐Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

    *Correspondences: Meng‐Zhu Lu (lumz@caf.ac.cn); Shu‐Tang Zhao (shutang@caf.ac.cn, Dr. Zhao is fully responsible for the distributions of all materials associated with this article)
  • Received:2021-07-07 Accepted:2021-11-22 Online:2022-01-01 Published:2022-01-25

Abstract: Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated PagSAG101a, the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar (Populus alba × Populus glandulosa) clone 84K and investigated its role in secondary xylem development. PagSAG101a was expressed predominantly in lignified stems and localized in the nucleus. Compared with non-transgenic 84K plants, transgenic plants overexpressing PagSAG101a displayed increased plant height, internode number, stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for PagSAG101a knock-out plants. Transcriptome analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem formation. In addition, the tandem CCCH zinc finger protein PagC3H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of PagSAG101a and mediate the regulation of xylem differentiation. Our results support that PagSAG101a, downstream of PagC3H17, functions in wood development.

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