J Integr Plant Biol ›› 2022, Vol. 64 ›› Issue (4): 843-843.DOI: 10.1111/jipb.13231

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  • 收稿日期:2021-10-05 接受日期:2021-01-26 出版日期:2022-04-01 发布日期:2022-04-20

CDC48B facilitates the intercellular trafficking of SHORT-ROOT during radial patterning in roots

Lihui Yang1,2,3†, Mingyue Zhu1,4,5†, Yi Yang1,4,5, Ke Wang1,4,5, Yulei Che1,4,5, Shurui Yang1,4,5, Jinxiang Wang6, Xin Yu7, Lixin Li8, Shuang Wu9, Klaus Palme2,5 and Xugang Li1,4,5*   

  1. 1 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Daizong Street 61, Tai'an 271018, China
    2 Institute of Biology II/Molecular Plant Physiology, Faculty of Biology, Albert‐Ludwigs‐University of Freiburg, Schänzlestrasse 1, Freiburg D‐79104, Germany
    3 Department of Genetics, Northwest Women's and Children's Hospital, Xi'an 710061, China
    4 Shandong Engineering Research Center of Plant‐Microbia Restoration for Saline‐alkali Land, Shandong Agricultural University, Daizong Street 61, Tai'an 271018, China
    5 Sino German Joint Research Center for Agricultural Biology, College of Life Sciences, Shandong Agricultural University, Daizong Street 61, Tai'an 271018, China
    6 State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources & College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510640, China
    7 Citrus Research Institute, Southwest University, Chongqing 400712, China
    8 Key Laboratory of Saline‐alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China
    9 FAFU‐UCR Joint Center and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

    †These authors contributed equally to this work.
    *Correspondence: Xugang Li; Contact : Yong Wang (wangyong@sdau.edu.cn)
  • Received:2021-10-05 Accepted:2021-01-26 Online:2022-04-01 Published:2022-04-20

Abstract: CELL DIVISION CONTROL PROTEIN48 (CDC48) is essential for membrane fusion, protein degradation, and other cellular processes. Here, we revealed the crucial role of CDC48B in regulating periclinal cell division in roots by analyzing the recessive gen1 mutant. We identified the GEN1 gene through map-based cloning and verified that GEN1 encodes CDC48B. gen1 showed severely inhibited root growth, increased periclinal cell division in the endodermis, defective middle cortex (MC) formation, and altered ground tissue patterning in roots. Consistent with these phenotypes, CYCLIND 6;1(CYCD6;1), a periclinal cell division marker, was upregulated in gen1 compared to Col-0. The ratio of SHRpro:SHR-GFP fluorescence in pre-dividing nuclei vs. the adjacent stele decreased by 33% in gen1, indicating that the trafficking of SHORT-ROOT (SHR) decreased in gen1 when endodermal cells started to divide. These findings suggest that the loss of function of CDC48B inhibits the intercellular trafficking of SHR from the stele to the endodermis, thereby decreasing SHR accumulation in the endodermis. These findings shed light on the crucial role of CDC48B in regulating periclinal cell division in roots.

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