J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (8): 722-729.DOI: 10.1111/jipb.12323

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Detection and fine-mapping of SC7 resistance genes via linkage and association analysis in soybean

Honglang Yan1†, Hui Wang1†, Hao Cheng1, Zhenbin Hu1,2, Shanshan Chu1, Guozheng Zhang1 and Deyue Yu1*   

  • 收稿日期:2014-08-25 接受日期:2014-12-19 出版日期:2014-12-22 发布日期:2014-12-22

Detection and fine-mapping of SC7 resistance genes via linkage and association analysis in soybean

Honglang Yan1†, Hui Wang1†, Hao Cheng1, Zhenbin Hu1,2, Shanshan Chu1, Guozheng Zhang1 and Deyue Yu1*   

  1. 1National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China
    2Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
  • Received:2014-08-25 Accepted:2014-12-19 Online:2014-12-22 Published:2014-12-22
  • About author:†These authors contributed equally to this work.
    *Correspondence: E-mail: dyyu@njau.edu.cn

被引次数

25

摘要： SMV is one of the most serious soybean diseases worldwide. A total of 19 SNPs associated with disease rate were identified via association analysis, and one of them was fine-mapped to an interval less than 200 kb using linkage analysis. Finally, three candidate genes were obtained within this region.

Abstract:

Soybean mosaic virus (SMV) disease is one of the most serious and broadly distributed soybean (Glycine max (L.) Merr.) diseases. Here, we combine the advantages of association and linkage analysis to identify and fine-map the soybean genes associated with resistance to SMV strain SC7. A set of 191 soybean accessions from different geographic origins and 184 recombinant inbred lines (RILs) derived from Kefeng No.1 (resistant) × Nannong 1138-2 (susceptible) were used in this study. The SC7 resistance genes were previously mapped to a 2.65 Mb region on chromosome 2 and a 380 kb region on chromosome 13. Among 19 single nucleotide polymorphisms (SNPs) detected via association analysis in the study, the SNP BARC-021625-04157 was located in the 2.65 Mb region, and the SNP BARC-041671-08065 was located near the 380 kb region; three genes harboring the SNPs were probably related to SC7 resistance. The resistance gene associated with BARC-021625-04157 was then fine-mapped to a region of approximately 158 kb on chromosome 2 using 184 RILs. Among the 15 genes within this region, one NBS-LRR type gene, one HSP40 gene and one serine carboxypeptidase-type gene might be candidate SC7 resistance genes. These results will be useful for map-based cloning and marker-assisted selection in soybean breeding programs.

 

Yan H, Wang H, Cheng H, Hu Z, Chu S, Zhang G, Yu D (2015) Detection and fine-mapping of SC7 resistance genes via linkage and association analysis in soybean. J Integr Plant Biol 57: 722–729 doi: 10.1111/jipb.12323

Key words: Association analysis, fine-mapping, linkage analysis, soybean, soybean mosaic virus

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