J Integr Plant Biol ›› 2015, Vol. 57 ›› Issue (9): 745-756.DOI: 10.1111/jipb.12336

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Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice

Dekai Wang1,2, Heqin Liu1,2, Sujuan Li1,2, Guowei Zhai1,2, Jianfeng Shao1,2 and Yuezhi Tao1,2*   

  • 收稿日期:2014-12-01 接受日期:2015-01-26 出版日期:2015-01-31 发布日期:2015-01-31

Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice

Dekai Wang1,2, Heqin Liu1,2, Sujuan Li1,2, Guowei Zhai1,2, Jianfeng Shao1,2 and Yuezhi Tao1,2*   

  1. 1Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
    2China State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
  • Received:2014-12-01 Accepted:2015-01-26 Online:2015-01-31 Published:2015-01-31
  • About author:*Correspondence: E-mail: yuezhitao8657@163.com

摘要： OsSHM1, an ortholog of Arabidopsis SHM1 in rice, was cloned and characterized. The osshm1 mutant showed a typical photorespiratory phenotype and was subjected to significant oxidative stress. Insight into the specific details of the photorespiratory pathway in rice will be beneficial to reduce photorespiration and enhance rice productivity.

Abstract:

Serine hydroxymethyltransferase (SHMT) is important for one carbon metabolism and photorespiration in higher plants for its participation in plant growth and development, and resistance to biotic and abiotic stresses. A rice serine hydroxymethyltransferase gene, OsSHM1, an ortholog of Arabidopsis SHM1, was isolated using map-based cloning. The osshm1 mutant had chlorotic lesions and a considerably smaller, lethal phenotype under natural ambient CO2 concentrations, but could be restored to wild type with normal growth under elevated CO2 levels (0.5% CO2), showing a typical photorespiratory phenotype. The data from antioxidant enzymes activity measurement suggested that osshm1 was subjected to significant oxidative stress. Also, OsSHM1 was expressed in all organs tested (root, culm, leaf, and young panicle) but predominantly in leaves. OsSHM1 protein is localized to the mitochondria. Our study suggested that molecular function of the OsSHM1 gene is conserved in rice and Arabidopsis.

 

Wang D, Liu H, Li S, Zhai G, Shao J, Tao Y (2015) Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice. J Integr Plant Biol 57:745–756 doi: 10.1111/jipb.12336

Key words: gene, molecular cloning, reactive oxygen species, rice, serine hydroxymethyltransferase

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