J Integr Plant Biol ›› 2023, Vol. 65 ›› Issue (6): 1383-1393.DOI: 10.1111/jipb.13457  cstr: 32098.14.jipb.13457

所属专题： Abiotic stress

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  • 收稿日期:2022-08-17 接受日期:2023-01-19 出版日期:2023-06-01 发布日期:2023-06-21

The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis

Yechun Hong1,2†, Yang Gao3†, Jia Pang2, Huazhong Shi4, Tingting Li3, Huiying Meng3, Dali Kong2, Yunjuan Chen2, Jian-Kang Zhu1,2* and Zhen Wang2,3*   

  1. 1. Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
    2. Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
    3. School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
    4. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA
    †These authors contributed equally to this work.
    *Correspondences:Jian-Kang Zhu(zhujk@sustech.edu.cn);Zhen Wang(wangzhen@ahau.edu.cn, Dr. Wang is fully responsible for the distributions of the materials associated with this article)
  • Received:2022-08-17 Accepted:2023-01-19 Online:2023-06-01 Published:2023-06-21

Abstract: Salt stress adversely impacts crop production. Several spliceosome components have been implicated in regulating salt stress responses in plants, however, the underlying molecular basis is still unclear. Here we report that the spliceosomal core protein SmEb is essential to salt tolerance in Arabidopsis. Transcriptome analysis showed that SmEb modulates alternative splicing of hundreds of pre-mRNAs in plant response to salt stress. Further study revealed that SmEb is crucial in maintaining proper ratio of two RCD1 splicing variants (RCD1.1/ RCD1.2) important for salt stress response. In addition, RCD1.1 but not RCD1.2 is able to interact with the stress regulators and attenuates salt- sensitivity by decreasing salt-induced cell death in smeb-1 mutant. Together, our findings uncovered the essential role of SmEb in the regulation of alternative pre-mRNA splicing in salt stress response.

Key words: alternative splicing, RCD1, salt stress, SmEb, spliceosome

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