Please wait a minute...

J Integr Plant Biol, 60 (5): 432-443, Research Article
A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust
Bing Wang1, Jinping Wei2, Na Song2, Ning Wang2, Jing Zhao2 and Zhensheng Kang2,3*
1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling 712100, China
2State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China
3China-Australia Joint Center for Abiotic and Biotic Stress Management, Northwest A&F University, Yangling 712100, China

*
Correspondence:
Email: Zhensheng Kang (kangzs@nwsuaf.edu.cn)
Abstract

NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30, from a cDNA library constructed from wheat (Triticum aestivum) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one‐hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C‐terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst. Silencing of TaNAC30 by virus‐induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H2O2. Finally, we overexpressed TaNAC30 in fission yeast and determined that cell viability was severely reduced in TaNAC30‐transformed cells grown on medium containing H2O2. These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat‐Pst interaction.

---------------------------------------------------
Received: 21 November 2017      Published: 18 December 2017
© 2018 Institute of Botany, Chinese Academy of Sciences
This Article
   Full Text
   Full Text (PDF)
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
PubMed
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22