J Integr Plant Biol

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Base editing in rice using nuclease‐deactivated CRISPR/Cas‐SF01

Meng Jia, Yangqing Xie, Chuang Wei, Hongzhi Wang, Li Xue, Chunyu Zou, Jian-Kang Zhu, Mugui Wang

doi: 10.1111/jipb.70265

Version of Record online: 14 April 2026

Adenine and cytosine base editing using dCas-SF01 and the 35S-CmYLCV-U6 composite promoter successfully introduced targeted base substitutions at multiple loci in rice, with average editing efficiency of 33.3%. Using the protospacer adjacent motif (PAM)-relaxed SF01-IKRR variant enabled base editing using 5'-NTN PAMs in rice.

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OmicsCanvas: A multi-omics platform for integration and visualization of epigenetic regulation

Zeyu Zhang, Zuoling Ma, Tian Hua, Xuqing Liu, Shengcai Xiang, Lianfeng Gu

doi: 10.1111/jipb.70258

Version of Record online: 12 April 2026

OmicsCanvas integrates diverse epigenomic and transcriptomic data into a unified framework, enabling users to visualize complex multi-omics patterns, generate publication-ready figures, and uncover regulatory relationships. This tool makes multi-omics analysis accessible to biologists without bioinformatics expertise.

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From recognition to proteolytic control: NLRs and metacaspases in plant antiviral immunity

Lin Liang, Yuhang Jiang, Mengrong Wang, Jian Ye

DOI: 10.1111/jipb.70259

Version of Record online: 12 April 2026

This review highlights how immune receptors and proteases work together to limit viral spread. This coordination connects detection to action and reveals new opportunities to engineer crops with stronger and broader resistance to viral diseases.

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SIB1-SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia

doi: 10.1111/jipb.70251

Version of Record online: 12 April 2026

SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR-BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.

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Pangenome analysis reveals the genetic mechanism underlying high-altitude adaptation in Qinghai–Xizang (Tibet) Plateau Rhododendron

Haoyang Zhou, Zhongping Xu, Fanhuang Zeng, Haiyu Sang, Zhenhua Liu, Miao Sun, Qiang Fu, Kaige Zhao, Daming Tan, Manzhu Bao, Shuangxia Jin, Xiuqun Liu

doi: 10.1111/jipb.70252

Version of Record online: 12 April 2026

Pan-genome analysis reveals that high-altitude Rhododendron species resist alpine cold stress by rapidly sensing and engaging the chilling response pathway and genes that directly and indirectly protect the plant from UV radiation. Heritable genomic features such as long terminal repeats contribute to the adaptive diversification of Rhododendron species.

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Towards an integrated molecular understanding of plant hormones

Louise Vilain, Marie-Laure Fauconnier, Manon Genva

DOI: 10.1111/jipb.70238

Version of Record online: 09 April 2026

This review offers a comprehensive overview of the nine plant hormones, delving into their biosynthesis, transport, signaling and crosstalk mechanisms. Because the complexity of plant hormonal control goes beyond these core elements, additional specific features are discussed. Lastly, this review highlights how fundamental insights drive hormonal-based applied developments.

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The mechanism of newly differentiated leaves of kale turning white in late autumn

Zhichao Gong, Yupeng He, Xinyuan Li, Zhiwei Zhang, Shuonan Wang, Jie Ren, Hui Feng

DOI: 10.1111/jipb.70250

Version of Record online: 02 April 2026

The newly differentiated leaves of ornamental kale turn white in late autumn because low temperature induces expression of the C-repeat binding factor gene BoCBF1. BoCBF1 activates expression of the F-box gene BoWl. BoWl interacts with the transcription factor BoARF1 to upregulate the transcription factor gene BoGLK1 and promote chloroplast development.

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Engineering the efficient Exo-Cas12i2 for MITE manipulation in rice

Nan Chai, Wuge Liu, Kangli Sun, Qian Qian, Yao-Guang Liu, Xianrong Xie, Qinlong Zhu, Jiantao Tan

doi: 10.1111/jipb.70254

Version of Record online: 02 April 2026

Exo-Cas12i2 v1, a fusion of the 5′ exonucleases T5E and PapE, facilitates editing of TA-rich regions and mediates deletions of large genomic fragments. Exo-Cas12i2 v1-driven MITE manipulation enables precise regulation of genes involved in gibberellin-mediated cell elongation and root ethylene responses to generate favorable agronomic traits.

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Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns

Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi

doi: 10.1111/jipb.70249

Version of Record online: 02 April 2026

Intron optimization of LbCas12a-RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double-strand break-free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research.

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Rewiring the SPX‐mediated signaling for climate‐resilient and nutrient‐efficient crops

Xuge Lou, Keke Yi

doi: 10.1111/jipb.70255

Version of Record online: 01 April 2026

This Commentary summarizes the original work by Liao et al. on the role of NITROGEN LIMITATION ADAPTATION (NLA) in cold tolerance, places it within the broader context of climate change and nutrient constraints, and discusses the engineered NLA^Δ12 allele and its implications for overcoming the trade-off between cold tolerance and phosphorus use efficiency.

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Metabolic engineering of rice endosperm for crocin biosynthesis

Lijuan Wang, Jianjie Gao, Wenhui Zhang, Yongdong Deng, Hao Zhang, Rihe Peng, Bo Wang, Jing Xu, Hongjuan Han, Zhenjun Li, Yu Wang, Cen Qian, Yongsheng Tian, Quanhong Yao

DOI: 10.1111/jipb.70246

Version of Record online: 01 April 2026

Crocins are carotenoids and are the main active ingredients of the precious medicinal plant saffron. Metabolic engineering was used to develop a rice germplasm, ‘crocin rice’, which biosynthesizes crocins and various other carotenoids, and is expected to be used to improve human nutrition and health.

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Coordinating nitrogen‐responsive root and shoot growth for enhanced nitrogen‐use efficiency

Xiaoming Zheng, Qian Qian

doi: 10.1111/jipb.70247

Version of Record online: 01 April 2026

The transcription factor WRINKLED1a coordinates nitrogen-responsive root and shoot growth in rice, improving nitrogen-use efficiency and yield stability.

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A conserved fungal Egh16‐like effector suppresses host defense by disrupting ATP binding of wheat MPK3

Jiangang Kang, Gang Niu, Yihui Liao, Daiying Xu, Huaijian Xu, Qing Yang, Daiyuan Sun, Kaili Duan, Yang Yang, Chenfang Wang, Qinhu Wang, Huiquan Liu, Jin-Rong Xu, Cong Jiang

DOI: 10.1111/jipb.70241

Version of Record online: 30 March 2026

Fusarium graminearum deploys the conserved effector Cee1 to interfere with wheat immune signaling. Upon entry into host cells, Cee1 associates with the ATP-binding domain of the immune kinase TaMPK3, impairing its activity and defense responses. This work highlights the ATP-binding pocket of plant kinases as a target for pathogen effectors.

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Fungal effector FolCP1b promotes infection by sequestering host subtilase SlSBT1 to protect apoplastic effectors from degradation

Zhengang Miao, Baoshan Wang, Limin Song, Wenxing Liang

DOI: 10.1111/jipb.70244

Version of Record online: 30 March 2026

Upon infection, the pathogen Fusarium oxysporum f. sp. lycopersici sends the decoy protein FolCP1b into the plant cell. FolCP1b binds to the subtilase defense enzyme SlSBT1, trapping it in the cytoplasm and pregenting it from reaching and destroying other fungal proteins outside the cell, thereby promoting infection.

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TADs, CGVs, and compartmentalization in genomes: Providing a new way for crop domestication and improvement

Qamar U. Zaman, Robert J. Henry, Zhihua Mu, Shuangqian Shen, Jie Luo, Rajeev K. Varshney

DOI: 10.1111/jipb.70240

Version of Record online: 30 March 2026

This review discusses how environmentally responsive modulation of 3D genome architecture may contribute to adaptive traits and crop improvement. In parallel, under unusual environmental conditions, cryptic genetic variations in plants play a role in adaptation, although how higher-order chromatin structure influences this remains unclear.

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12‐hydroxylauric acid, a novel growth regulator, promotes plant organ development

Yan Peng, Mengling Zhang, Zengdong Tan, Hongxiang Lou, Bao Yang, Yuting Zhang, Zhicong Zhao, Ruyi Fan, Haiyan Fan, Jie Chen, Xuan Yao, Shaoping Lu, Hu Zhao, Liang Guo, Kede Liu

DOI: 10.1111/jipb.70243

Version of Record online: 30 March 2026

The pleiotropic gene BnaA09.CYP78A9 regulates silique length, seed number per silique and seed size in rapeseed (Brassica napus). 12-hydroxylauric acid, the catalytic product of the CYP78A9 enzyme, enhances fruit and seed size across multiple crops.

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Systemic stomatal immunity: A rapid gate-closing circuit unveiled in plants

Fengling Wu, Fanrong Peng, Jing Guo, Ming Wu, Jianguo Wu

doi: 10.1111/jipb.70245

Version of Record online: 24 March 2026

This Commentary highlights a recent study discovering Systemic Stomatal Immunity, a rapid, targeted immune program, filling a research gap by showing local challenge triggers distal stomatal closure within hours.

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FcMAPK4-phosphorylated FcNOR activates FcERF5 to promote fig fruit softening through activation of FcPG12 expression

Yuan Wang, Zhiyi Fan, Yining Wang, Alexander Vainstein, Yuexuan Qiu, Yinan Sun, Huiqin Ma

doi: 10.1111/jipb.70239

Version of Record online: 23 March 2026

In Ficus carica fruit, the MAP kinase FcMAPK4 phosphorylates the NAC transcription factor FcNOR, which cooperates with ethylene response factor FcERF5 to activate the pectin degradation gene FcPG12, thereby promoting rapid softening of fig fruit.

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The AcLFYL1-AcCSE module enhances kiwifruit resistance to Pseudomonas syringae pv. actinidiae by regulating cell density and lignin biosynthesis

Wenpeng Song, Yantao Wu, Yangyang Li, Wei Li, Yue Huang, Junyang Yue, Pu Liu, Xueren Yin, Yongsheng Liu, Faming Wang, Songhu Wang, Wei Tang

DOI: 10.1111/jipb.70232

Version of Record online: 23 March 2026

Pseudomonas syringae pv. actinidiae causes destructive kiwifruit canker. Resistant kiwifruit shows higher lignin and cell density. AcLFYL1 directly activates AcCSE to promote lignin biosynthesis and cell density, enhancing canker resistance, providing genetic targets for resistance breeding.

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PanGraphRNA: An efficient and flexible bioinformatics platform for graph pangenome-based RNA-seq data analysis

Yifan Bu, Zhixu Qiu, Wen Sun, Yishui Han, Yifan Liu, Jing Yang, Minggui Song, Zenglin Li, Songyu Liu, Yuzhou Zhang, Chuang Ma

doi: 10.1111/jipb.70231

Version of Record online: 19 March 2026

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Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications

Mingming Wu, Maohong Cai, Rongrong Zhai, Jing Ye, Guofu Zhu, Faming Yu, Shenghai Ye, Xiaoming Zhang

doi: 10.1111/jipb.70217

Version of Record online: 19 March 2026

Pentatricopeptide repeat proteins coordinate nucleus-organelle communication by modulating RNA metabolism within chloroplasts and mitochondria. This review highlights how they control critical processes like photosynthesis, seed development, fertility restoration, and stress survival in crops, and explores their potential as programmable tools for RNA manipulation in organelles.

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Metabolite‐mediated plant immunity: From traditional defensors to artificial elicitors

Jiajun Tang, Weiwei Xie, Yunjiang Cheng, Alisdair R. Fernie, Feng Zhu

DOI: 10.1111/jipb.70225

Version of Record online: 17 March 2026

This review summarizes the roles of plant metabolites in immunity and pathogen-induced reprogramming; it also explores nanoscience, de novo domestication, and artificial intelligence for the development of next-generation elicitors.

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Unlocking the genetic basis of quantitative hybrid sterility in rice

Xin Gou, Ruiying Liu, Yaoguang Liu, Yongyao Xie

doi: 10.1111/jipb.70229

Version of Record online: 17 March 2026

This commentary summarizes hybrid sterility models in plants, with an emphasis on a recent study that addresses the genetic basis of the RIS/RIA-RID-RIR system underlying S44-mediated hybrid sterility between Oryza longistaminata and indica rice, revealing a novel killer-protector-target model integrated with modifiers that regulate reproductive isolation.

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An efficient transformation and gene editing system for Lilium species

Yajie Zhao, Yixuan Li, Jinxin Shi, Yutong Chen, Jingwei Wei, Jinlong Li, Yiqing Wang, Dingyi Liu, Dong Jing, Shuo Long, Manman Zhang, Junwei Tang, Jian Wu

doi: 10.1111/jipb.70237

Version of Record online: 17 March 2026

A transformation system exploits lily bulb scale propagation and de novo formation of bulblets, integrating tissue culture with non-tissue culture approaches. Through optimized scale propagation and disinfection treatments, it achieves efficient stable gene overexpression and editing in lily.

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Transcriptional adapter ADA2 regulates yield and end‐use quality through liquid–liquid phase separation in wheat

Xiaobang Zhang, Ruijie Zhang, Yuhan Zhang, Tao Yang, Longfei Wu, Yuan Cao, Jun Xiao, Jigang Li, Xin Gao, Xinyou Cao, Jie Liu, Mingming Xin, Yuan Zong, Zhongfu Ni, Qixin Sun, Yingyin Yao

doi: 10.1111/jipb.70227

Version of Record online: 16 March 2026

The transcriptional adapter ADA2 interacts with the histone acetyltransferase GCN5 to regulate starch and storage protein content in wheat grains through histone acetylation. ADA2 undergoes phase separation and is modulated by GCN5, affecting histone acetyltransferase activity and gene transcription. An elite ADA2-B haplotype correlates with improved grain size.

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OsCPN10a, cooperating with OsCPN20 and OsHSP60‐3B negatively regulate ABA signaling and enhance seed storability in rice

Sufeng Liao, Yidong Wei, Yongsheng Zhu, Kunyang Li, Ting Chen, Shuai Zhao, Fangxi Wu, Jinlan Wang, Pengbo Yu, Hongguang Xie, Liping Chen, Qiuhua Cai, Huaan Xie, Jianfu Zhang

doi: 10.1111/jipb.70230

Version of Record online: 16 March 2026

Abscisic acid signaling homeostasis is essential for seed storability. The molecular chaperone OsCPN10a enhances rice seed storability by forming a trimeric chaperone complex with OsCPN20-OsHSP60-3B that attenuates abscisic acid signaling via direct interaction with OsPYL10-OsABIL1, thereby maintaining starch integrity and offering a promising target for crop improvement.

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Developing a robust multiplex CRISPR/Cas12i3‐5M system for trait stacking in soybean

Wenxin Lin, Haijie Wu, Huaqin Kuang, Xiangchi Feng, Mengyan Bai, Fanghui He, Ruixuan Liang, Yizhi Zeng, Meina Li, Fanjiang Kong, Baohui Liu, Yuefeng Guan

doi: 10.1111/jipb.70233

Version of Record online: 16 March 2026

The high-efficiency multiplex gene editing technology based CRISPR-Cas12i3-5M is capable of simultaneously editing 13 target sites in soybean, and was used to generate germplasm with high oleic acid content and no beany flavor.

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Constitution and function of intergenic genome regions in cultivated versus annual wild soybean in China

Xiaoshuai Hao, Xinyang Hu, Yongpeng Pan, Qi Zhang, Ran Ou, Fenfen Jiang, Fangdong Liu, Jiaoping Zhang, Wubin Wang, Guangnan Xing, Junyi Gai, Jianbo He

doi: 10.1111/jipb.70223

Version of Record online: 16 March 2026

Three-case restricted two-stage multi-locus multi-allele genome-wide association study identified main-effect genes and detected the regulatory effects of intergenic loci on genes, enabling comprehensive dissection of the genic-intergenic genetic system for quantitative traits and providing a strategy for studying their genetic basis.

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Correction to “The miR172a–ERF416/413 module regulates soybean seed traits”

DOI: 10.1111/jipb.70228

Version of Record online: 15 March 2026

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Synergistic engineering of Casδ nuclease for robust genome editing

Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen

doi: 10.1111/jipb.70222

Version of Record online: 15 March 2026

A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.

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SlGRF1 mediates gibberellin signaling to control cut‐budding in tomato

Yaping Xu, Yinhuan Xie, Huimin Jia, Qianqian Li, Xinqin Liu, Ting Ma, Zhaobo Lang, Qingfeng Niu

doi: 10.1111/jipb.70219

Version of Record online: 15 March 2026

Upon wounding, cytokinin increases to initiate callus formation. Gibberellin enhances this, but later blocks bud formation by suppressing SlGRF1. Wounding-induced protein SlGRF1 triggers buds via NAM1, EPF4, and ER2. Hormonal shifts control cut-budding phases.

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GSK3 regulates DOG1L4 to enhance pre‐harvest sprouting resistance in wheat

Huixue Dong, Danning Yang, Yu He, Qian Chen, Xiaojiang Guo, Mengping Cheng, Maolian Li, Zhongwei Yuan, Jirui Wang

doi: 10.1111/jipb.70234

Version of Record online: 15 March 2026

GLYCOGEN SYNTHASE KINASE 3 (GSK3), a negative regulator of brassinosteroid signaling, phosphorylates and stabilizes the seed dormancy protein DOG1L4, which in turn activates ABSCISIC ACID INSENSITIVE 5 (ABI5) to enhance seed dormancy. GSK3 also phosphorylates ABI5. This dual-target mechanism suggests a strategy for controlling seed dormancy and germination in crops.

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Two mycorrhiza-responsive MADS transcription factors, OsMADS61 and OsMADS26, regulate both direct and mycorrhizal nitrate transport pathways

Yuhan Ren, Congfan Yang, Haoyan Ji, Kun Xie, Haoran Mao, Dechao Zeng, Lingxiao Wang, Shuangshuang Wang, Guohua Xu, Aiqun Chen

DOI: 10.1111/jipb.70224

Version of Record online: 12 March 2026

In rice, the transcription factors OsMADS26 and OsMADS61 orchestrate direct and symbiotic nitrate uptake pathways via transcriptionally activating the nitrate transporter genes OsNPF4.5 and OsNAR2.1/NRT2.2 by recognizing the CrAG cis-element in their promoters, consequently enhancing nitrogen acquisition and utilization efficiencies during arbuscular mycorrhizal symbiosis.

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LpMPK6 regulates leaf color in perennial ryegrass by inhibiting LpMYBR1-modulated transcription of LpNRT1.5

Liaoliao Ye, Xiuhua Tang, Zhijian Cao, Cun Wang, Jinwei Yang, Zhiquan Qiang, Tao Qin

DOI: 10.1111/jipb.70226

Version of Record online: 12 March 2026

In perennial ryegrass, the MAP kinase LpMPK6 phosphorylates the transcription factor LpMYBR1 to suppress transcription of the nitrate transporter gene LpNRT1.5, thereby inhibiting root-to-shoot nitrate transport and causing leaf chlorosis under nitrogen deficiency.

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SlARF11–SlGATA5 stimulates tomato leaf angle by feedback regulation with brassinosteroid signaling

Qihui Wang, Ruoxuan Wang, Xi Wang, Junfeng Luo, Qiongqiong Zhang, Xin Liu, Jing Jiang

DOI: 10.1111/jipb.70215

Version of Record online: 12 March 2026

Leaf angle in tomato is regulated by a regulatory module involving the non-canonical auxin response factor SlARF11, which interacts with SlGATA5, facilitating SlGATA5 binding to the promoters of genes involved in brassinosteroid signaling, cell elongation, and lignin deposition.

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CasY7: An optimized Cas12i system for enhanced genome editing in monocot crops

Dating Zhong, Yanxiao Dong, Hong Pan, Yanan Fu, Yining Zhao, Shuting Ruan, Wencong Yu, Yiwen Wang, Qianlan Yin, Yanfei Zhang, Yisen Huang, Jiaqi Shen, Hongling Zhang, Yuxuan Wu, Jieting Xu, Yuming Lu

DOI: 10.1111/jipb.70181

Version of Record online: 11 March 2026

The Cas12i-derived nuclease CasY7 system achieved high editing efficiency in rice, maize, and wheat, notably enabling multiplex editing. This establishes CasY7 as a robust CRISPR tool for crop improvement.

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HSP101-encoding NEO-TETRAPLOID RICE FERTILITY GENE 1 regulates tapetum development through interaction with SAPK2 in polyploid rice

Lichong Cao, Weicong Huang, Hang Yu, Sanglin Liu, Jianmin Yin, Zijun Lu, Jinwen Wu, Xiangdong Liu

doi: 10.1111/jipb.70218

Version of Record online: 11 March 2026

In neo-tetraploid rice, the NTRF1–SAPK2 heat shock protein–kinase module coordinates abscisic acid signaling with reactive oxygen species homeostasis to ensure timely tapetal programmed cell death and subsequent pollen maturation, providing a resource for the genetic improvement of polyploid rice.

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The SAG101–NRG1 branch of TIR signaling activates phenylalanine-derived SA biosynthesis in Nicotiana benthamiana

Lu Xu, Mingsong Wu, Yanan Liu, Xin Li, Yuelin Zhang

DOI: 10.1111/jipb.70214

Version of Record online: 11 March 2026

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Transcriptome-wide association study identifies the ZmbZIP89–ZmSAUR21 module as a key regulator of root growth and drought tolerance in maize

Tianze Zhu, Houmiao Wang, Yunyun Wang, Xiaoyi Yang, Shuai Fang, Wei Li, Wenye Rui, Baoqing Wang, Yimei Tian, Dan Sun, Haoxin Gao, Zefeng Yang, Chenwu Xu, Pengcheng Li

DOI: 10.1111/jipb.70220

Version of Record online: 11 March 2026

The maize Small Auxin-Up RNA gene ZmSAUR21 is activated by the transcription factor ZmbZIP89 and regulates root development, providing a direct target for breeding maize with stronger, deeper root systems, that are more resilient to drought.

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Efficient CRISPR/Cas‐SF01 genome editing tools with high editing efficiency in allotetraploid oilseed rape

Mengyu Hao, Meili Zhou, Fei Pan, Tiantian Liu, Yilin Li, Nan Su, Ayub Ashfaq, Miaoying Song, Hui Wang, Wenxiang Wang, Jia Liu, Chao Li, Li Fu, Ping He, Qiong Hu, Desheng Mei, Hongtao Cheng

DOI: 10.1111/jipb.70221

Version of Record online: 09 March 2026

An efficient protoplast system was used to test gene editing and Cas-SF01 was developed for gene editing in oilseed rape and used to create plants with multilocular siliques and male sterility, with high precision and no off-target edits. Cas-SF01 recognizes more flexible DNA targets than CRISPR-Cas9.

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