Abstract: Calli could be induced from various parts of flowers of Crocus sativus cultured in vitro. The appropriate media for steady induction of style-stigma-like structures were MS or N6+NAA 5 mg/L. +6-BA 5 mg/L. The style-stigma-likestructures resemble the natural ones in both appearance and inner structures. Both pigments of picrocrocin and safranal, which are known to accumulate only in the stigmas and styles under natural condition and are important for the high quality of saffron, were extracted and identified from the style-stigma-like structures and calli from explants of flowers.

Key words: Crocus sativus, In vitro, Style-stigma-like structure, Pigment