Study on High-Frequeney Plant Regeneration from Monocell Suspension ,Cultures of Astragalus grubovii

Abstract

Abstract: Callus colony PL1 possessing the potential for a high frequency of plant regeneration (997.1%) was obtained from selected brown and green calli of Astragalus grubovii explants in subcultures. A relatively homogeneous cell suspension line was established from the callus colony in liquid Bx medium containing NO3- and NH4+ at a NO3-/NH4+ of 10:1 on a rotary shaker. Calli having a high frequency of plant regeneration (968.9%) were obtained from the the cell suspension line in monocell cultures.

Key words: Astragalus grubovii, Monocell, High-frequency, Plant regeneration

An Li-jia, Zhang Jun-min,Li Feng-xia,Luo Xi-ming,He Meng-yuan and Hao Shui. Study on High-Frequeney Plant Regeneration from Monocell Suspension ,Cultures of Astragalus grubovii[J]. J Integr Plant Biol., 1993, 35(Suppl.): -.

[1]	LI Hui Fen, LI Xu Gang, LIU Xiang, CHANG Tuan Jie, XIAO Gui Fang, ZHU Zhen. Maize Transformation of cry1Ac3 Gene and Insect Resistance of Their Transgenic Plants [J]. J Integr Plant Biol., 2002, 44(6): 684-688.
[2]	WANG Zhe-Zhi, ZHANG Su-Feng and HU Zheng-Hai. Isolation,Culture and Plant Regeneration of Protoplasts from an Embryogenic Callus Tissue of Gossypium hirsutum [J]. J Integr Plant Biol., 1998, 40(3): -.
[3]	Zhang Xiang-qi, Wang Xian-ping, An Li-jia and Chu Jing-hua. Plant Regeneration from Protoplasts of Medicago lupulina [J]. J Integr Plant Biol., 1996, 38(3): -.
[4]	Chen Xi-wen, Chen De-fu, Zhou Pu-hua and Li Zong-dao. Protoplast Culture and Plant Regeneration of Ramie [J]. J Integr Plant Biol., 1996, 38(2): -.
[5]	Wang Guan-lin and Fang Hong-jun. Establishment of Somaclones and Their Chromosomal Variation in Allooctoploid Triticum- Agropyron Hybrids [J]. J Integr Plant Biol., 1995, 37(6): -.
[6]	Wang Ying, Huang Min-ren, Chen Dao-ming, Xu Nong, Wei Zhi-ming and Xu Zhi-hong. Plants Regenerated from Mesophyll Protoplasts of Cottonwood New Clone [J]. J Integr Plant Biol., 1995, 37(5): -.
[7]	Zhang Xiang-qi, Wang Xian-ping, An Li-jia, Fan Song-hua and Xiang Wei. Plant Regeneration from Protoplasts of Talinum paniculatum [J]. J Integr Plant Biol., 1995, 37(10): -.
[8]	Zhang Lan-ying, Chen Ru-zhu and Li Geng-guang. High Frequency Callus Formation and Plant Regeneration from Leaf Protoplasts of Pepino (Solanum muricatum L.) [J]. J Integr Plant Biol., 1994, 36(Suppl.): -.
[9]	Dai Chao-xi and Sun Shun-di. Culture of Cotyledon and Hypocotyl Protoplasts from True Potato Seedlings in Solanum tuberosum L. [J]. J Integr Plant Biol., 1994, 36(9): -.
[10]	Zhang Hong, Tian Hui-qin and Li Jun-ming. Plant Regeneration from Cell and Protoplast Cultures of Triticum aestivum-Haynaldia villosa Hybrid [J]. J Integr Plant Biol., 1994, 36(6): -.
[11]	Liao Zhao-zhou, Chen Ming-zhou, Liao Qiao-xia, Yan Qiu-sheng and Zhang Xue-qin. Plant Regeneration from Protoplasts of Sugarcane [J]. J Integr Plant Biol., 1994, 36(5): 0-.
[12]	Ding Ai-ping, Wang Hong-fan and Cao Yu-fen. Protoplast Culture and Plant Regeneration of Malus pumila [J]. J Integr Plant Biol., 1994, 36(4): -.
[13]	Zhang Lan-ying, Li Geng- guang, Chen Ru-zhu and Li Kai-lian. Protoplast Culture and Plantlet Regeneration from Hypocotyls of Brassica campestris var. parachinesis [J]. J Integr Plant Biol., 1994, 36(2): -.
[14]	Xia Guang-min, Li Zhong-yi and Chen Hui-min. Plant Regeneration from Cell Supension Derived Protoplasts of Heracleum moellendorffii [J]. J Integr Plant Biol., 1993, 35(9): -.
[15]	Li Xue-bao,Xu Zhi-hong,Wei Zhi-ming and Bai Yong-yan. Somatic Embryogenesis and Plant Regeneration from Protoplasts of Cowpea (Vigna sinensis) [J]. J Integr Plant Biol., 1993, 35(8): -.

Study on High-Frequeney Plant Regeneration from Monocell Suspension ,Cultures of Astragalus grubovii

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments