Abstract: This paper deals with the fossil plants from the Yuntaiguan formation located between Hunan and Hubei province. There are six genera and ten species (containing a new genus and five new species) to be discussed here, such as Protolepidodendron scha-ryanum Krejci, Barrandeina dusliana (Krejci) Stur, B. contigua sp. nov., B. lixianensis sp. nov., B. multistriata sp. nov., B. laxa sp. nov., Drepanophycus spinaeformis Goepp., “Protopteridium”? minutum Halle, Psilophyti4es sp. and Longostachys latisporophyllus gen. et sp. nov., etc. The appearance of this fossil flora concerned here can be correlated to the Protolepidodendron scharyanum-Barrandeina dusliana Assemblage. Among these fossil plants from Yuntaiguan formation, both the Protolepidodendron scharyanum and Barrandeina dusliana are also the index fossils, the prior was wide spread during the Middle Devonian in whole world and the other was only found at the late Middle Devonian in Europe. In the Yuntaiguan formation the genus Barrandeina containing no less than five species and, the other taxa were also found from the Middle or the late Middle Devonian. So that this formation should be referred to the late Middle Devonian in age and this conclusion corresponds with the result of spore-pollen analysis in the Xiushuigou formation, Xiongzi county, which is similar to the Yuntaiguan formation and has been referred to the Middle Devonian. In this paper, only four species of Barrandeina and one species of Longostachys have been described, those of the former have not been found before in China. The authors think; this will be significant on studying fossil plants and in comparison with the age of the strata.