J Integr Plant Biol ›› 1994, Vol. 36 ›› Issue (4): -.

• Research Articles •    

Fluorescence Decaying Kinetics and Photodamage of Quinone-Reconstituted D1/D2/Cytochrome b559 Complex

Tang Chong-qin, Kuang Ting-yun, Yu Zhen-bao, Lu Rong-he, Peng De-chuan, Tang Pei-song and Li Cheng-yong   

Abstract: Photosystem Ⅱ reaction center D1/D2/Cytochrome b559 complex loses its bound secondary electron acceptor QA and QB during isolation and purification. The artificial plastoquinone can reconstitute with the complex. The reconstitution of decyl-plastoquinone (DPQ) with D1/D2/Cytochrome b559 complex results in a decrease of the fraction of the two long lived fluorescence decay components (24 ns and 73 ns) coupled with photochemical activities to the total fluorescence yields, as well as a decrease of the total fluorescence intensity and a blue-shift of maximum emission wavelength. These results suggest that as the electron acceptor of reduced Pheo, DPQ restricts the charge recombination of P680+ Pheo-, and the two long lived fluorescence decay components (24 ns and 73 ns) come from the recombination. Although DPQ reconstitution has little effect on the susceptibility of Chi a to photodamage, β-carotene can easily be photodamaged after DPQ reconstitution. This is probably related to the physiological function of β-carotene.

Key words: D1/D2/Cytochrome b559 complex, Photodamage, Fluorescence lifetime

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