J Integr Plant Biol ›› 1992, Vol. 34 ›› Issue (10): -.

• Research Articles •    

Plant Regeneration from Protoplasts of Actinidia chinensis Planch.

Xiao Zun-an, Shen De-xu and Lin Bo-nian   

Abstract: Protoplasts isolated from cotyledon callus line of A14N7 of Actinidia Chinensis Planch. were cultured in the improved NN-69 medium. First division of regenerated cells occurred during 7–10 days of culture, and percentage of the cell division was about 10% at day 20. The best result of protoplast culture was achieved when protoplasts were cukured in liquid medium at a density of 5× 104/ml, About 4 months, procoplast-derived calli were transferred stepwisely onto differentiation media where they developed into green compact calli, from which the perfect plants were regenerated.

Key words: Actinidia chinensis, Protoplasts, Plant regeneration

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