J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (8): 719-726.DOI: 10.1111/j.1744-7909.2009.00845.x

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Analyses of Sexual Reproductive Success in Transgenic and/or Mutant Plants

Cristiane P. G. Calixto1,2, Gustavo H. Goldman3 and Maria Helena S. Goldman1*   

  1. 1 Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo 14040-901, Brazil
    2 PPG Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo 14049-900, Brazil
  • Received:2009-04-27 Accepted:2009-05-07 Published:2009-08-17
  • About author:3 Departamento de C. Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo 14040-903, Brazil
    *Author for correspondence Tel: +55 16 3602 3702; Fax: +55 16 3602 4886; E-mail: mgoldman@ffclrp.usp.br


The pistil, the female reproductive organ of plants, is a key player in the success of sexual plant reproduction. Ultimately, the production of fruits and seeds depends on the proper pistil development and function. Therefore, the identification and characterization of pistil expressed genes is essential for a better understanding and manipulation of the plant reproduction process. For studying the function of pistil expressed genes, transgenic and/or mutant plants for the genes of interest are used. The present article provides a review of methods already exploited to analyze sexual reproductive success. It intends to supply useful information and to guide future experiments in the study of genes affecting the pistil development and function.

Calixto CPG, Goldman GH, Goldman MHS (2009). Analyses of sexual reproductive success in transgenic and/or mutant plants. J. Integr. Plant Biol. 51(8), 719-726.

Key words: knockout mutant plants, overexpression transgenic plants, pistil expressed genes, plant sexual reproduction, reproductive success, silenced transgenic plants.

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