J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (Suppl.): -.

• Research Articles •    

High Frequency Callus Formation and Plant Regeneration from Leaf Protoplasts of Pepino (Solanum muricatum L.)

Zhang Lan-ying, Chen Ru-zhu and Li Geng-guang   

  • Published:1994-12-31

Abstract: Protoplasts were isolated from young leaves of two cultivars of pepino, Solanum muricatum L., and cultured on SCM, LCM and M-K8p media for 1–2 d respectively. Protoplasts became enlarged and produced cell wall, then initial division was observed. Division rate of 85 % in LCM medium was calculated one week after culturing. The plate efficiency of small clone (PE) was higher than that of P Ⅰ (47.9%) and P Ⅱ (62.7%). Ability of shoot formation in calli derived from protoplast culture as well as genetypes of pepino; was different among that in dilution media. The best result in shoot formation (30. 1%) was obtained in regeneration medium supplemented with IAA 0. 1 mg/L, and ZT 2.0 mg/L. Shoots were transferred onto MS+ IAA 0. 2 mg/L to complete rooting following which the plantlets were successfully grown in soil.

Key words: Solanum muricatum, Protoplast culture, Plant regeneration

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22