J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (7): -.

• Research Articles •    

Cytological Observation on Megasporogenesis and Embryo Sac Development of Regenerated Ovule in Hyacinth

Lu Wen-liang,Qian Nan-fen,K. Enomoto and Y. Fukunaga   

Abstract: The morphogenesis of regenerated ovule and cytological changes of its megasporogenesis and embryo sac development were studied. Results showed as follows: 1. the differentiation of the regenerated ovule had followed a normal process in the order of inner integument , outer integument and then funiculus. But the form of the regenerated ovules in vitro was quite different from that of ovule in vivo. Most of the regenerated ovules were orthotropous and hemianatropous , only a few were anatropous which are the same with that in vivo. 2. the megasporogenesis and the embryo sac development also had normal cytological process ,and the Polygonum type-embryo sac consisted of one egg, two synergids , one central cell and three antipodals could be seen in mature regenerated ovule. These ex-perimental results make clear that the regenerated ovule differentiated directly from explant could accomplish the complex processes of megasporogenesis and embryo sac development. By this fact ,authors infer that once the differentiation of ovule primordium, the complex biochemical programs for the megasorogenesis and embryo sac development can be controlled by the ovule itself and need no more information from flower bud and /or plant.

Key words: Hyacinthus orientalis, Regenerated ovule in vitro, Megasporogenesis, Embryo sac Development

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