J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (6): -.

• Research Articles •    

Anther Culture of Naked Oat and the Establishment of Its Haploid Suspension Cell

Sun Jing-san (C. S. Sun), Lu Tie-gang and M. R. Sōndahl   

Abstract: This paper reported the production of haploid plants through anther culture in naked oat (Arena nuda). Calluses were induced from anthers of naked oat placed on various culture media. MS medium with 4% sucrose, 1% activated charcoal and no hormones gave the highest initiation frequencies (14.7%) of anther callus among media tested. Twelve green plants and one albino plant have been regenerated from anther calluses. Cytological examination of mitotic rooot tip ceils from three green anther plants showed that two of the plants were haploid (2n=3x=21) and one was diploid (2n=6x=42). The cell suspension cultures were established from pollen friable calluses in liquid medium. The suspension cells were cytologically stable during one year subcultures. Most of the ceils examined were haploid.

Key words: Avena nuda, Anther culture, Haploid, Suspension culture

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