Please wait a minute...
J Integr Plant Biol, 1992, 34 (4): -, Research Article
The Relation Between Anaerobic Switch-off Nase Activity and ATP/ADP Ratio in Ceils of Azospirillum brasilense
Li Jiu-di, Wang Ji-wen and Li Yong-xing
doi:
Abstract
Anaerobic switch-off of Nase activity in A. brasilense "Yu-62" cultured aerobically in liquid medium with glutamic acid as N source was described. CCCP inactivated Nase rapidly. Western blotting experiment demonstrated that the molecular basis of inactivation of Nase was the modification of the Fe protein subunit. The anaerobic condition reduced ATP/ADP ratio in the cells and switched-off the Nase activity. The restoration of aerobic condition gave rise to rapid increase of ATP/ADP ratio and Nase activity. CCCP reduced ATP/ADP ratio rapidly. Metronidazole, a low potential electron acceptor, inhibited Nase activity but has no effect on modification of Fe protein, however, caused a slight change of ATP/ADP ratio. Our experiments demonstrated that the change of ATP/ADP ratio in cells was a signal trigging the modification of Fe protein.
--------------------------------------------------------------------------------------------------------------------
   Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Li Jiu-di
   Articles by Wang Ji-wen
   Articles by Li Yong-xing
PubMed
   Articles by Li Jiu-di
   Articles by Wang Ji-wen
   Articles by Li Yong-xing
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22