J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (2): -.

• Research Articles •    

Isolation and Characterization of a Chinese Aneurolepidium Cell Line Resistant to Hydroxyproline

Chen Hui, Kuang Bai-jian and Wang Jing-ju   

Abstract: A hydroxyproline (HYP) resistant cell line of Chinese aneurolepidium (Aneurolepidium chinense (Trin.) Kitag. ) was isolated under selection pressure of 20 mmol/L HYP. Comparison of the free amino acid pool levels in the cell line with that of donor showed substantial accumulation of proline (6.6 × ). Enzyme examination indicated that γ-glutamyl kinase controlling proline biosynthesis in HR20-8 cell line had 2.5 times as much activity as that of the donor. Exogenous L-proline inhibited the enzyme activities both in the HR20-8 and the donor by the same rate of 30% at 100 mmol/L. The responses of HR20-8 cell line to NaC1, PEG and cold temperature (5 ℃) were also compared with those of donor and the former exhibited remarkably increased tolerance to the tested stress condition. The results showed that changes of γ-glutamyl kinase property exhibited the phenomenon of extra accumulation of proline which might favor the increased tolerance to NaC1, PEG and cold temperature in the resistant cell line.

Key words: Chinese aneurolepidium, Variant line: Tissue culture, Proline

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q