Please wait a minute...
J Integr Plant Biol, 1996, 38 (5): -, Research Article
Pollen-Mesophyll Protoplast Fusion and Hybrid Plant Regeneration in Nicotiana
Lu Ping, Zhou Chang and Yang Hong-yuan
Isolated pollen protoplasts of Nicotiana tabacum L. N364 Km+ were fused with mesophyll protoplasts of N. rustica L. using PEG-high Ca/pH method. The cells resulted from fusion between immature (early-middle bicellular) pollen protoplasts and mesophyll protoplasts could divide to produce microcalli and regenerated plantlets when cultured in a selection KM8p medium containing 50 g/L kanamycin. Four plantlets were regenerated. The isoenzyme patterns of leaf peroxidases of these plantlets had bands characteristic of both parents. Root-tip squash showed that the gameto-somatic hybrids had the expected triploid chromosome number. Aside from these kanamycin-selected plantlets, six of the twenty-one plantlets that had not undergone selection were also evidenced to be gameto-somatic hybrids.
   Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Lu Ping
   Articles by Zhou Chang
   Articles by Yang Hong-yuan
   Articles by Lu Ping
   Articles by Zhou Chang
   Articles by Yang Hong-yuan
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail:
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q