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J Integr Plant Biol, 1999, 41 (12): -, Research Article
Protein Electrophoretic Analysis of Amyloplast and Cytoplasm Ribosomes from Lotus Cotyledon
SUN De-Lan, WANG Hong and DONG Yun-Zhou
doi:
Abstract
Amyloplasts and cytoplasmic ribosomes in cotyledon cells of lotus (Nelvmbo nucifeva Gaertn. ) have been observed on the basis of morphology. Isolation of these ribosomes by centrifugation through 30% to 55% (W/V) sucrose density gradient resulted in three bands of amyloplasts ribosomes and four bands of cytoplasmic ribosomes. The authors used these ribosomes bands for SDS-PAGE electrophoresis to analyse ribosomes of proteins. The patterns of SDS-PAGE between cytoplasmic ribosomes of proteins and amyloplasts ribosomes of proteins were different. The amyloplasts ribosomes of proteins showed 26 kD and 23 kD bands, and the cytoplasmic ribosomes of proteins showed 65 kD band. The analysis of electrophoretic patterns of the cytoplasmic ribosomes of proteins showed that there was a newly synthesized ribosomes protein with 19 kD molecular weight in 18 to 20 days after fertilization.
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