J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (11): -.

• Research Articles •    

Relationship Between nifZ and the Synthesis of P cluster in Nitrogenase MoFe Protein of Azotobacter vinelandii

HUANG Ju-Fu, WANG Dao-Yong, DONG Zhi-Gang, WANG Zhi-Ping, LI Jia-Ge and WANG Yao-Ping   

Abstract: In comparison with OP MoFe protein from wild type strain Azotobacter vinelandii Lipmann, the C2H2-reduction activity and atom ratio of Fe to Mo of △nifZ MoFe protein from a nifZ deletion strain of A. vinelandii were remarkably decreased. FeMoco, which were extracted from these two proteins under the same condition, were almost similar to each other in activity and metal composition, and the circular dichroism (CD) spectra of these proteins were significantly different from each other. In the visible region except 540 750 nm, the △ε at 380 - 540 nm of △nifZ MoFe protein decreased and had a peculiar sharp negative peak around 430 nm; and in the ultraviolet region, the peaks at 208 nm and 222 nm were higher than those of OP MoFe protein. △nifZ MoFe protein could be crystallized in a suitable concentration of PEG 8000 and MgCl2, the size of crystals and amount of precipitation seemed to be related to the above-mentioned negative peaks. The results showed that △nifZ of Azotobacter vinelanclii might be related to the synthesis of P-cluster, rather than to that of FeMoco, which resulted in its conformation, stability and process of crystallization.

Key words: nifZ of Azotobacter vinelandii, Synthesis of P-cluster in △nifZ MoFe protein, Protein conformation and crystallization

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