J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (11): -.

• Research Articles •    

Pathogen resistant Transgenic Plant from Dwarfing Rootstock of Cherry by Introducing Antibacterial Polypeptide Genes

FANG Hong-Jun, WANG Guan-Lin, WANG Huo-Xu, JIA Shi-Rong, DONG Yun-Zhou and TANG Yi-Xiong   

Abstract: A shoot regeneration system of high frequency from shoot of Chinese cherry ( Prunus pseudocerasus Lindl. ) dwarfing rootstock was established and 19 transformed plants were obtained from the dwarfing rootstock by introducing gus gene and antibacterial polypeptide genes, using Agrobacteriuan tumefaciens (Smith et Townsend) Corm as mediator. The results of Southern blot analysis proved that antibacterial polypeptide gene was integrated into the cherry genome. The results of tumor test, X-gluc reaction and bacterial-resistant test of leaf extract against Agrobacterium tumefaciens C58 indicated that antibacterial polypeptide gene may be expressed efficiently in the transformed plant. The tube shoot was characteristic in its resistance to root nodule disease. This study on the characteristics of germ line transformation of shoot meristem suggested that it was a highly efficient transformation system.

Key words: Cherry, Antibacterial polypeptide gene, Shoot transformation

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