J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (11): -.

• Research Articles •    

Enhancement of Rice Disease Resistance by Two Antifungal Protein Genes

FENG Dao-Rong, XU Xin-Ping, WEI Jian-Wen, LI Bao-Jian, YANG Qi-YUN and ZHU Xiao-Yuan   

Abstract: Hasmid pGB12 containing a rice basic chitinase gene RC24 and an alfalfa fl-l, 3-glucanase gene β-1,3- Glu and p35H containing hygromycin phosphotransferase gene hpt were simultaneously bombarded into an elite indica rice (Oryza sativa L. ssp. indica ) variety-" Qisiruanzhan" grown in South China. 17 independent lines co-harboring RC24 and β-1,3-Glu were obtained by Southern blot. Northern blot analysis revealed substantial variations among these transfonnants. The introduced RC24 and β-1,3-Glu were stably inherited and expressed in the second and third generations. To the five isolates of Magnaphortha grisea varieties, evaluation of leaf blast of the second generation showed a different degree of resistance. And every progeny of 18 R1 transgenic rice plants showed resistance to the three dominant isolates. Based on bioassay of leaf blast with R2 transgenic rice plants and data from the molecular analyses, ten homologous transgenic lines were primarily obtained, which exerted higher inhibitor efficiency on the growth of Rhizoctonia solani Kiilm.

Key words: Transgenic rice plants, Basic chitinase gene, β-1, 3-glucanase gene, Resistance to rise blast, Resistance to Rhizoctonia solani

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