J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (10): -.

• Research Articles •    

Reactivation of Partially Metalloclusterdeficient MoFe Protein by Rhenium-containing Reconstituent Solution

HUANG Ju-Fu, WANG Zhi-Ping, WANG Dao-Yong, DONG Zhi-Gang and LI Jia-Ge   

Abstract: When the reduced MoFe protein from Azotobacter vinelandii Lipmann was treated with ophenanthroline and air, an inactive protein partially deficient in both FeMoco and P-cluster could be obtained. After incubating the treated protein with a reconstituent solution containing Re2OT, ferric homocitrate, Na2S and dithiothreitol, which had no circular dichroism (CD) signal, the ultraviolet and visible CD spectra, the C2H2 and H+ -reduction activity of the incubated protein were significantly recovered. However, the spectra were somewhat different from those of the reduced MoFe protein. The results showed that: 1) in the incubated protein solution there was possibly a new recombined ReFe protein besides the intact MoFe protein which was not destroyed by the treatment with o-phenanthroline and air; 2) it might be possible that both ReFe protein and MoFe protein exhibited similar ability of nitrogen fixation, although they were somewhat different in structure.

Key words: Inactive MoFe protein, Re-containing reconstituent solution, Re-containing nitrogenase reconstituted in vitro, Azotobacter vinelandii

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