Abstract: Seven crystalline substances were isolated from alcohol extract of the root bark of Acanthopanax gracilistylus W. W. Smith, by column chromatography on silica gel. They were identified by various physical and chemical methods as stearic acid, (d)-sesamin, β- sitosterol, syringin, β-sitosterol glucoside, eleutheroside B1 and 16 α-hydroxy-(–)-kauran-19-oic acid. The 16 α-hydroxy-( — )-kauran-19-oic acid was isolated from species of Acan- thopanax for the first time.