J Integr Plant Biol. ›› 1983, Vol. 25 ›› Issue (4): -.
• Research Articles •
Li Shu-jun, Cai Jiau-ping, Xiao Jian-ping and Xiu Ya-nan
The basic mechanisms of phosphorylation of chloroplasts and mitochondria are identical. The identity may be proved by membrane combination. There are two ways to get the combination as shown in figure 1. One way is, as previously reported, to combine deficient membranes from chloroplasts with ctista membranes from mitochondria and the reconstituted membranes thus obtained greatly enhance photophosphorylated activities. The other way, i.e., to combine deficient crista membranes with thylakoid membranes, has also been successful, as shown in this paper. The reconstituted membranes obtained in this way can carry out oxidative phosphorylation in the dark as well as shown in Table 2. There are some relationship between the ATP formation from oxidative phosphorylation of reconstituted membranes and the protein of deficient crista membranes added, as shown in Table 3. When the quantity of combined chloroplast membranes is kept constant, the amount of ATP formation varies, within certain limits, with the amount of deficient crista membranes as shown in Table 3. But the reconstituted oxidative phosphorylation activity of membranes formed by combinating thylakoid with deficient crista membranes is lower than reconstituted photophosphorylation activity of combination in the opposite direction, i.e. by combinating deficient thylakoid membranes and crista membranes of mitochondria (compare Table 4 and 3).
Li Shu-jun, Cai Jiau-ping, Xiao Jian-ping and Xiu Ya-nan. Reconstitution of the Functional Membranes from Chloroplasts with the Deficient Crista Membranes from Mitochondria[J]. J Integr Plant Biol., 1983, 25(4): -.
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