Abstract: This work studies the whole process of fertilization in Glycine max. The results are summarized as follows: 1. The type of ripened pollen grain of soybean was two-cell type. The generative cell was divided mitotically into two spermatids within the pollen tube. 2. In the 6th hour after self-pollination, the pollen tube entered into the embryo sac and released two sperms. Before the fusion of the male and female nuclei, the cytoplasmic sheath of the spermatids falled off. The cytoplasm of the male gamete did not fuse with that of the egg cell. 3. In the 6th hour after self-pollination, one spermatid nucleus come in contact with the egg nucleus and the other with the secondary nucleus, The contact of the sperimatid nucleus with the egg nucleus was a little earlier than that with the secondary nucleus. 4. The spermatid nucleus entered into the egg nucleus; the chromatic granules of the spermatid despiralized. After the complete fusion of the spermatid nucleus with the egg nucleus, the egg nucleus was darkly stained and the chromonemata increased, afterward the male nucleus appeared. 5. In the 28th hour after self-pollination, the zygote begun the first mitotic division. It took about 20 hours to fuse the male and female gametes, and to form the zygote untile before first mitotic division. It means that the zygote dormancy stage of soybean was about twenty hours. 6. In the 7th hour after self-pollination, the spermatid nucleus fused with the secondary nucleus. The process was similar to that of the spermatid nucleus with the egg nucleus. The chromatic granules gradually despiralized within the secondary nucleus, and the male nucleoli appeared. The velocity of the fusion of the spermatid nucleus with the secondary nucleus was faster than that of the other spermatid nucleus with the egg nucleus. 7. In the 10th hour after self-pollination, the secondary nucleus begun the first mitotic division. 8. The fertilization of soybean was the premitotic type.