Abstract: Callus could be induced from the cotyledon segments of Cucumis redo L. in Miller’s medium supplement with high concentration of kinetin (10–15 mg/L) or with NAA 2 mg/L plus kinetin 0.5 mg/Ll. The calli induced by NAA plus kinetin were much different from those by kinetin alone. The former was loose and soft. While the latter was tight and gradually developed new buds. Isoenzymes of glucose-6-phosphate dehydrogenases, isocitrate dehydrogenases, malate dehydrogenases and glutamate dehydrogenases were detemineted at 5, 10, 15, 20, 25 and 30 d after inoculation. The results showed that both type of calli contained four dehydrogenases during their growth period. This indicates that the pentose phosphate pathway, TCA cycle and glutamate metabolism are present in calli of both types during their growth. The isoenzymes of glucose-6-phosphate dehydrogenases and malate dehydrogenases in shoots developing calli are different from those in shootless ones.