J Integr Plant Biol. ›› 1980, Vol. 22 ›› Issue (4): -.

• Research Articles •    

Effect of Different Ammonium Salts on Formation of Maize Pollen Embryoid

Ming Shu-hua   

Abstract: There are more than 80% embryoids among the induced masses in anther culture; different ammonium salts produced different effects on the formation of embryoids, and the effect of NH4H2PO4 is better than the others, within the range of the concentrations used. When the combinations of KNO3 With NH4H2PO4 act as the nitrogen source the frequencies of formation of embryoids and calli increase with the increase of NO3–/NH4+ ratio, at NO3–/NH4+ = 6:1, the frequency is the highest, but at 8:1, it decreases. In the treatments of lower total nitrogen (22.5–37.5 mN/L), the induction frequency is higher (3%–12%) than those of higher total nitrogen (45–60 mN/L). It has also been discussed that the different materials responded to the same ammonium salt, that the same material responded to different NO3–/NH4+ ratio.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22