Abstract: This report describe the organogenesis of Populus davidiana × P. bolleana Loucne hybrid leaf explant from plantlet in vitro and the effects of different factors on its organogenesis. It was found that shoot induction of lower leaf segments with petiole was easier than those of the middle and top segments. When leaf explants were in- cubated on a MS midium with eytokinin (zeatin l×l0-6 M–2×10-6 M or benzyladenine 2 × 10-6 M) and auxin (IAA 4 × 10-8 M–1 × 10-6 M or NAA, NAAoxy 4 × 10-8 M–4 × 10-7 M), the frequeney of shoot differentiation would be 70%–100%. Kinetin had no activity on shoot formation and 2, 4-D showed serious inhibition. ABA, GA3, and Ethrel all prevented shoot production. Since higher frequency of shoot differentiation could be obtained, a lot of shoots were produced cneach leaf explant and shoots could be proliferated by axillary buds, so this system could be used for rapid propagation.