Abstract: The present paper deals with the study of factors affecting the frequency of induction of Triticale pollen callus in anther culture. The following experimental results have been obtained: 1. The N6 medium and B5 medium were superior to MS medium in the production of pollen callus, and the difference between N6 medium and B5 medium was not obvious. 2. When 6 to 12 per cent of sucrose was added in medium, the induction frequency of pollen callus was higher, however, this higher frequency decreased with the concentration of sucrose down to 3 per cent. 3. It was found that there was no regultr correlation between the frequency of the formation of the pollen callus and concentrations of 2,4-D (0.5 ppm, 2 ppm, 5 ppm and 10 ppm) in medium. 4. Induction frequency of pollen callus increased apparently when active carbon (0.5 per cent) was added. 5. The liquid medium was capable of improving yields of the pollen callus. 6. It is not important for the formation of the pollen callus whether the cultures were kept in light or in dark. 7. Before inoculation the spikes were inserted into a bottle with N6 medium solution and pretreated at 3–5℃ for 3 days, the frequency of callus formation may be efficiently increased in comparison with those treated in water. When N6 medium was supplemented with 2,4-D (2 ppm), NAA (2 ppm) and kinetin (0.5 ppm), either used singly or in combination, the frequency of callus formation was not obviously increased.