J Integr Plant Biol. ›› 1978, Vol. 20 ›› Issue (2): -.

• Research Articles •    

The Production of Wheat Pollen Embryo and the Influence of Some Factors on Its Frequency of Induction

Pan Jing-li and Gao Gong-hong   

Abstract: The pollen plants developed directly from wheat pollen grains by two ways: (1) The uninucleate pollen divided many times and developed into mature pollen embryos, which then grew into plantlets; (2) The globular embryo grew further and formed irregular small callus which in turn differentiated rapidly into plantlets on the same medium without any transplanting. 2. Of all basal mediums N6 medium is considered to be the best one. 3. If the spike of wheat is treated with the centrifuge at 2000 rev./min, for 20 minutes before placing on the medium, the frequency of plantlets developed directly from pollen grains is, greatly increased. 4. The extraneous hormone was found to be beneficial to the embryogenesis of the wheat pollen embryos. The best result was obtained from the anthers cultured continuously at 7–10 ℃ for 120 hours on medium added with 2:6 mg/l IAA and kinetin after inoculation. Under these conditions the frequency of induction increased to 6.2%.

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