J Integr Plant Biol. ›› 1977, Vol. 19 ›› Issue (2): -.

• Research Articles •    

Studies on Maize Anther Culture

Institute of Maize Research, Kwangsi Chuang Autonomous Region, Experimental Station of Dong Bei Wang Commune, Hai-dian, Peking and Peking Institute of Botany, Academia Sinica   

Abstract: Callus has been induced from maize anther culture in 30 different materials and haploid seedlings have been differentiated in 15 of them. When the anther with the uninucleate pollen grains at the middle stage was inoculated, the induction' frequency of haploid callus (or embryoid) was the highest, reaching 3.25%, and this was 3.92 times of increase in comparison with the pollen grains at the stage of side-located uninucleus. The culture medium for inducing callus is N6 + K 2, 4-D 2 mg/l + 1 mg/l + CH 500 mg/l, and that for differentiation is N6 + K 1 mg/l + CH 500 mg/l.2-or 4-celled pollen' grains and multicellular masses have been observed in squashed anthers. Root tip squash revealed that the chromosome number being 10 and this proved that the haploid plantlet was derived from the pollen grain. The haploid plantiers have been transplanted in the soil and then treated with colchicine solution at the concentration of 0.025-0.05% for doubling the chromosome number and the plantlets grew normally. Now the first batch of transplanted plants is in heading and silking stage.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q