J Integr Plant Biol. ›› 1973, Vol. 15 ›› Issue (2): -.

• Research Articles •    

Cytological Studies on the Androgenesis of Triticale

Sun Chin-san, Wang Chin-chü and Chu Zhih-ching   

Abstract: This article deals with a study of the method in inducing pollen plants fromcultured anthers and cytological observations on the in vitro androgenesis of Triticale. The results obtained are summarized as follows: 1. Cell divisions of the uninucleate pollen grains and development of the am-phihaploid plants of Triticale can be readily obtained from anthers cultured on a basicmedium (slightly modified MS medium) supplemented with an appropriate amount of coconut milk and auxins (2,4-D, NAA). 2. The capacity of callus production varied greatly in different Triticalestrains. The pollens in the anthers of non-callus producing strains is potentially capable in producing embryoids, but the embryoids produced by them degenerate in anearly stage of development. 3. A higher proportion of the planted anthers producing shoots can be obtained, if the anthers containing embryoids had been transfered at a suitable time intothe medium for differentiation before the embryoidal cells developed into callus. 4. The two nuclei formed by the first division of the uninucleate microsporeare similar in appearance, they, however, generally differ in physiological functions. One of the nuclei having the nature of generative nucleus may divide and producefree nuclei which are eventually excluded in the course of embryoid development; the other having the nature of vegetative nucleus undergoes normal mitoses andtherefore plays an essential role in the embryoid formation. 5. In the differentiating or meristematic cells the starch accumulation is generallyrare, while in the cells surrounding them there are numerous starch grains.

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